{"id":"W4220863266","doi":"10.21203/rs.3.rs-1347437/v1","title":"Oleic acid based experimental evolution of Bacillus megaterium yielding an enhanced P450 BM3 variant","year":2022,"lang":"en","type":"preprint","venue":"Research Square","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval","funders":"","keywords":"Bacillus megaterium; Monooxygenase; Cofactor; Chemistry; Directed evolution; Enzyme; Cytochrome P450; Biochemistry; Stereochemistry; Biology; Mutant; Bacteria; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002832799,0.0004257559,0.000322954,0.000219015,0.0001563876,0.0004586002,0.0003152373,0.0003273286,0.0007523019],"category_scores_gemma":[0.0004166708,0.0001512113,0.0003110319,0.0004352199,0.0001979527,0.0001563546,0.000487239,0.0007097229,0.0003045906],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003190627,"about_ca_system_score_gemma":0.000207934,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007263698,"about_ca_topic_score_gemma":0.0007953395,"domain_scores_codex":[0.9997267,0.00005724938,0.00004315195,0.00005684174,0.00008014212,0.00003591948],"domain_scores_gemma":[0.9998173,0.000038759,0.00005506254,0.00003748285,0.00002681462,0.00002461216],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001029134,0.00006444091,0.0003728627,0.00004927393,0.0000147565,0.0001039197,0.00002457358,0.0003364689,0.995587,0.0001047134,0.00004726803,0.003191901],"study_design_scores_gemma":[0.00002424887,0.0003513743,0.002560339,0.00001234768,0.00003621064,0.0003068543,0.0000378355,0.002458077,0.9911245,0.00004676732,0.00302974,0.00001183172],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9906846,0.0003977242,0.00665246,0.0001652613,0.0000372869,0.00006356582,0.000457809,0.0001331584,0.0014081],"genre_scores_gemma":[0.9858991,0.0003956856,0.01030588,0.00006014243,0.000007270668,0.00005782032,0.001245639,0.00009841047,0.001930087],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007523019,"threshold_uncertainty_score":0.002516687,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03559247144802812,"score_gpt":0.359732712885456,"score_spread":0.3241402414374279,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}