{"id":"W4220962256","doi":"10.1038/s42003-022-03149-7","title":"Engineered Campylobacter jejuni Cas9 variant with enhanced activity and broader targeting range","year":2022,"lang":"en","type":"article","venue":"Communications Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":47,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Japan Society for the Promotion of Science; Japan Agency for Medical Research and Development","keywords":"Cas9; Genome editing; CRISPR; Biology; Campylobacter jejuni; Genome engineering; Endonuclease; Genome; Guide RNA; Computational biology; DNA; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001420133,0.0001106343,0.0001136636,0.00004022329,0.000251326,0.00000912006,0.0003311835,0.00006144563,0.00004141031],"category_scores_gemma":[0.00003227863,0.000105402,0.00002654536,0.00009413352,0.0001012985,0.000002449517,0.0005715163,0.0001768415,0.000001295534],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001503527,"about_ca_system_score_gemma":0.00003194484,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004615237,"about_ca_topic_score_gemma":0.00005252962,"domain_scores_codex":[0.9993432,0.0001304948,0.0001046798,0.0002117906,0.00003430804,0.0001754827],"domain_scores_gemma":[0.9990959,0.0000354965,0.00004228549,0.0007465716,0.00003511371,0.00004464935],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00005473956,0.00008264596,0.000937113,0.00000841459,0.00007151442,0.000001017463,0.0002241304,0.0005249092,0.9937374,0.000366084,0.0002116942,0.003780327],"study_design_scores_gemma":[0.004542854,0.002433086,0.02509487,0.00002427205,0.0001724131,0.0004964388,0.001665628,0.008114859,0.3914503,0.0002804289,0.5639146,0.001810327],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9654111,0.00367336,0.02889354,0.0006493856,0.00008362369,0.0002152034,0.00005552052,0.00002994945,0.0009882986],"genre_scores_gemma":[0.9942957,0.0004302424,0.004673521,0.0001001514,0.00003762382,0.00007846532,0.000231041,0.00001658196,0.0001367162],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.6022872,"threshold_uncertainty_score":0.4298167,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01019485543248507,"score_gpt":0.3000077223080717,"score_spread":0.2898128668755866,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}