{"id":"W4220962256","doi":"10.1038/s42003-022-03149-7","title":"Engineered Campylobacter jejuni Cas9 variant with enhanced activity and broader targeting range","year":2022,"lang":"en","type":"article","venue":"Communications Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":47,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Japan Society for the Promotion of Science; Japan Agency for Medical Research and Development","keywords":"Cas9; Genome editing; CRISPR; Biology; Campylobacter jejuni; Genome engineering; Endonuclease; Genome; Guide RNA; Computational biology; DNA; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001812573,0.0003465227,0.0002436198,0.0001608958,0.0001359688,0.0003284859,0.0002646004,0.0003825416,0.001192394],"category_scores_gemma":[0.0001905692,0.00008866042,0.000164001,0.0001840574,0.0002457731,0.0001465929,0.0001746459,0.0004606724,0.000294635],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004356131,"about_ca_system_score_gemma":0.0002611029,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001188076,"about_ca_topic_score_gemma":0.001350656,"domain_scores_codex":[0.9998521,0.00001468343,0.00001850419,0.00003968058,0.00004822074,0.00002676356],"domain_scores_gemma":[0.999887,0.00001968657,0.00003063927,0.00001417545,0.00001754206,0.00003098462],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006880679,0.0000188506,0.0000802903,0.00002196024,0.000004003329,0.00009204591,0.000007445164,0.0002180636,0.9984119,0.0001707146,0.0000572987,0.0008485119],"study_design_scores_gemma":[0.00001185072,0.0001191168,0.0005704921,0.000002031866,0.00000875663,0.0003066482,0.00001065769,0.001493574,0.995039,0.00002959368,0.002400455,0.00000777217],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9867212,0.0004492835,0.009853693,0.0001634848,0.00007919948,0.00004457571,0.0004175663,0.0002067168,0.002064326],"genre_scores_gemma":[0.9897476,0.0001170411,0.006758683,0.0000321484,0.000005270662,0.00001490984,0.0003851192,0.0000376012,0.002901538],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001192394,"threshold_uncertainty_score":0.003988922,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01019485543248507,"score_gpt":0.3000077223080717,"score_spread":0.2898128668755866,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}