{"id":"W4220967414","doi":"10.1021/acscentsci.1c01570","title":"A Synthetic Gene Library Yields a Previously Unknown Glycoside Phosphorylase That Degrades and Assembles Poly-β-1,3-GlcNAc, Completing the Suite of β-Linked GlcNAc Polysaccharides","year":2022,"lang":"en","type":"article","venue":"ACS Central Science","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canada's Michael Smith Genome Sciences Centre; University of British Columbia","funders":"Canadian Institutes of Health Research; Office of Science; Natural Sciences and Engineering Research Council of Canada; Government of Canada","keywords":"Glycoside hydrolase; Glycogen phosphorylase; Biology; Chitin; Biochemistry; Gene; Computational biology; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001618764,0.001038215,0.000464846,0.0006230165,0.0001581473,0.0004320963,0.0004673895,0.0004641254,0.001197871],"category_scores_gemma":[0.0002047022,0.0002289758,0.000559057,0.00078678,0.0004057884,0.0002353704,0.0003269238,0.0008640223,0.001118609],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003728651,"about_ca_system_score_gemma":0.0004984359,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000565405,"about_ca_topic_score_gemma":0.0009888093,"domain_scores_codex":[0.9997746,0.0000187951,0.00001386456,0.00007658215,0.00007137649,0.00004479035],"domain_scores_gemma":[0.9998866,0.00002614207,0.00002872886,0.00001613332,0.00001537279,0.00002701721],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004940264,0.00004770583,0.0001321633,0.00004708318,0.00000651429,0.00008631806,0.00001585675,0.000103574,0.9950227,0.000265165,0.00006710505,0.00415646],"study_design_scores_gemma":[0.00001343868,0.000269948,0.00132313,0.000007679125,0.00003955237,0.0004293365,0.00002437011,0.0007833443,0.9917272,0.000135062,0.005235487,0.00001130496],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8369154,0.001471781,0.1431188,0.0004178983,0.0002174792,0.000461451,0.006748426,0.001444929,0.009203807],"genre_scores_gemma":[0.8849391,0.002822367,0.06947632,0.0002467856,0.00005335091,0.0003794211,0.02091943,0.0003350917,0.020828],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001197871,"threshold_uncertainty_score":0.00400728,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01348332340297573,"score_gpt":0.223087526616457,"score_spread":0.2096042032134813,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}