{"id":"W4220967414","doi":"10.1021/acscentsci.1c01570","title":"A Synthetic Gene Library Yields a Previously Unknown Glycoside Phosphorylase That Degrades and Assembles Poly-β-1,3-GlcNAc, Completing the Suite of β-Linked GlcNAc Polysaccharides","year":2022,"lang":"en","type":"article","venue":"ACS Central Science","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canada's Michael Smith Genome Sciences Centre; University of British Columbia","funders":"Canadian Institutes of Health Research; Office of Science; Natural Sciences and Engineering Research Council of Canada; Government of Canada","keywords":"Glycoside hydrolase; Glycogen phosphorylase; Biology; Chitin; Biochemistry; Gene; Computational biology; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003583545,0.0001295069,0.0001204673,0.00004731558,0.0005262814,0.00008459221,0.0004520803,0.00003674286,0.00004946482],"category_scores_gemma":[0.0001167759,0.0001024893,0.00004713027,0.0003308656,0.0004014943,0.00003606173,0.0004530844,0.0001153576,6.284607e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001733115,"about_ca_system_score_gemma":0.0001431475,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005077501,"about_ca_topic_score_gemma":0.000009569341,"domain_scores_codex":[0.9986314,0.00009294999,0.0001953397,0.0004210306,0.0002896969,0.0003696306],"domain_scores_gemma":[0.9993666,0.00002855591,0.0001580857,0.0003106747,0.00003305074,0.0001029746],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004349359,0.00004689276,0.01589454,0.00001379355,0.0000103942,0.000001555909,0.000303284,0.00003306583,0.9806843,0.0004129586,0.0001294649,0.00242629],"study_design_scores_gemma":[0.0001553079,0.0001221719,0.03321698,0.00001343383,0.00001763407,0.00006086472,0.0003658913,0.0001987464,0.9605372,0.00004171389,0.005125728,0.0001443483],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9946528,0.003248072,0.0001325957,0.001444198,0.0001837518,0.0002364148,0.00002225615,0.00002182599,0.00005805867],"genre_scores_gemma":[0.9981359,0.000724935,0.0002973941,0.0004777641,0.00007208945,0.00002437707,0.00002919997,0.00001349976,0.0002248058],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02014709,"threshold_uncertainty_score":0.4179394,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01348332340297573,"score_gpt":0.223087526616457,"score_spread":0.2096042032134813,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}