{"id":"W4221037108","doi":"10.1038/s41467-022-29202-x","title":"Guide RNAs containing universal bases enable Cas9/Cas12a recognition of polymorphic sequences","year":2022,"lang":"en","type":"article","venue":"Nature Communications","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":47,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; University of Alberta","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Seoul National University; Government of Canada","keywords":"CRISPR; Computational biology; Cas9; Biology; Guide RNA; Genetics; Gene; Genome editing","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002859737,0.0004718353,0.000418348,0.0002748482,0.0003675189,0.0006378769,0.0004920179,0.0007694191,0.001626387],"category_scores_gemma":[0.0003923284,0.0003168502,0.0003124305,0.0002531542,0.00060063,0.0003531582,0.0006428383,0.001181041,0.0009204171],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000447761,"about_ca_system_score_gemma":0.0003994767,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007200849,"about_ca_topic_score_gemma":0.001821078,"domain_scores_codex":[0.9995382,0.0000529383,0.00004135176,0.0001507964,0.0001587515,0.00005804489],"domain_scores_gemma":[0.9997365,0.00004744214,0.00008034807,0.00005397094,0.00003630119,0.000045422],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004609513,0.00001650906,0.0001571835,0.00006001124,0.000007735359,0.00006946596,0.00002422565,0.0002556152,0.9902942,0.001382696,0.0001493086,0.007537048],"study_design_scores_gemma":[0.000005586286,0.00007232615,0.0003653708,0.000004829071,0.000008246112,0.000302176,0.0000100299,0.0009119466,0.9886587,0.0002119593,0.009439331,0.000009543878],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7061246,0.004115285,0.2699955,0.0006298528,0.0002900233,0.000357015,0.001189391,0.00344416,0.01385425],"genre_scores_gemma":[0.8418039,0.00148071,0.1455424,0.000244657,0.00003031375,0.0001110533,0.0008049161,0.0002507436,0.009731371],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001626387,"threshold_uncertainty_score":0.005440772,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01972781225922498,"score_gpt":0.3158736340998226,"score_spread":0.2961458218405976,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}