{"id":"W4221079362","doi":"10.1021/acschembio.1c00894","title":"Cryo-EM of the Yeast V <sub>O</sub> Complex Reveals Distinct Binding Sites for Macrolide V-ATPase Inhibitors","year":2022,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"ATP Synthase and ATPases Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"University of Toronto; Canada Research Chairs; Canadian Institutes of Health Research; Government of Ontario","keywords":"Bafilomycin; ATPase; V-ATPase; Endosome; Small molecule; Biochemistry; Biology; Binding site; Enzyme; Cell biology; Chemistry; Biophysics; Intracellular; Autophagy","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008084327,0.000299606,0.0001903151,0.0001486348,0.0004464352,0.0004045895,0.0004357099,0.0004757862,0.001256823],"category_scores_gemma":[0.0001772765,0.0002060789,0.0001946878,0.0001583763,0.0003065521,0.0003362943,0.0002874413,0.0008525723,0.0003046537],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005333635,"about_ca_system_score_gemma":0.0002433233,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004219982,"about_ca_topic_score_gemma":0.003597663,"domain_scores_codex":[0.9999398,0.000004730545,0.000002879797,0.00001822848,0.0000151493,0.00001924246],"domain_scores_gemma":[0.999902,0.00002900311,0.0000205552,0.000007775589,0.00001828666,0.00002251461],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000563851,0.00009609881,0.003995718,0.0001894135,0.00008723211,0.0009356983,0.0003249983,0.002968031,0.9842956,0.001305192,0.001149378,0.004088861],"study_design_scores_gemma":[0.0001057208,0.0004062599,0.09672394,0.0001504672,0.0001674287,0.002645449,0.001530191,0.03356535,0.8388079,0.001268003,0.02453445,0.00009482954],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9907487,0.001365726,0.003266863,0.0003385236,0.0000305849,0.000008524215,0.0004033752,0.00007140206,0.003766228],"genre_scores_gemma":[0.992407,0.001156459,0.003309976,0.0001954781,0.000008008397,0.00001445108,0.001272706,0.00006308651,0.001572804],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004219982,"threshold_uncertainty_score":0.008390844,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02468224693952399,"score_gpt":0.2935378236935354,"score_spread":0.2688555767540114,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}