{"id":"W4223427248","doi":"10.3390/molecules27082434","title":"Developing CRISPR/Cas9-Mediated Fluorescent Reporter Human Pluripotent Stem-Cell Lines for High-Content Screening","year":2022,"lang":"en","type":"article","venue":"Molecules","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Canadian Institutes of Health Research","keywords":"CRISPR; Induced pluripotent stem cell; Cas9; Genome editing; Green fluorescent protein; Biology; High-content screening; Reporter gene; Embryonic stem cell; Cell biology; Gene knockin; Gene; Genetics; Computational biology; Gene expression; Cell","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002104954,0.0002283042,0.0001960599,0.00007584066,0.0003121869,0.00003472644,0.0002307514,0.00007348664,0.00001747241],"category_scores_gemma":[0.00002446118,0.00023964,0.0001396197,0.00008456149,0.00002633874,0.000002619136,0.0003020448,0.0001037156,0.000001534364],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003979038,"about_ca_system_score_gemma":0.00005127086,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004317589,"about_ca_topic_score_gemma":0.00002092583,"domain_scores_codex":[0.9984953,0.00004162457,0.0003909478,0.000481542,0.0002154039,0.0003751742],"domain_scores_gemma":[0.9993303,0.0000127038,0.0001213045,0.00034773,0.0001020794,0.00008591606],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005561409,0.00005633744,0.0003417625,0.00005836303,0.00006745291,0.00002598257,0.00007279953,0.003371317,0.9921113,0.000115773,0.001814192,0.001909147],"study_design_scores_gemma":[0.001025951,0.0003560304,0.002072323,0.00001805803,0.00004001123,0.00002948358,0.0003960161,0.0006315876,0.9545333,0.00003381103,0.04043594,0.0004274236],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7446506,0.00141344,0.2526798,0.0003420071,0.0003118964,0.0004492075,0.00006035186,0.00004350727,0.00004921273],"genre_scores_gemma":[0.9871221,0.00003868634,0.01034334,0.0004375188,0.0002113897,0.00029356,0.0006800062,0.00006345567,0.000809991],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2424715,"threshold_uncertainty_score":0.9772236,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03035190781570126,"score_gpt":0.288474008727896,"score_spread":0.2581221009121947,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}