{"id":"W4223427248","doi":"10.3390/molecules27082434","title":"Developing CRISPR/Cas9-Mediated Fluorescent Reporter Human Pluripotent Stem-Cell Lines for High-Content Screening","year":2022,"lang":"en","type":"article","venue":"Molecules","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Canadian Institutes of Health Research","keywords":"CRISPR; Induced pluripotent stem cell; Cas9; Genome editing; Green fluorescent protein; Biology; High-content screening; Reporter gene; Embryonic stem cell; Cell biology; Gene knockin; Gene; Genetics; Computational biology; Gene expression; Cell","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009752882,0.0005744808,0.0006514579,0.0006608558,0.000260604,0.0006271139,0.0008640502,0.0007056218,0.00213945],"category_scores_gemma":[0.0006944987,0.0004045247,0.0005278856,0.0004507659,0.0002399707,0.0004168412,0.0004174421,0.0008152117,0.001944426],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003214684,"about_ca_system_score_gemma":0.000407048,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005833046,"about_ca_topic_score_gemma":0.001060458,"domain_scores_codex":[0.9992263,0.0001085137,0.0001116326,0.0001322484,0.0003568004,0.00006450848],"domain_scores_gemma":[0.9995891,0.0001033539,0.00008261948,0.00007657422,0.0001051793,0.00004310584],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003924619,0.00004077694,0.0002635432,0.00008392051,0.0000115334,0.0001059009,0.00002420693,0.0006467565,0.9919801,0.000324133,0.0003103258,0.006169466],"study_design_scores_gemma":[0.00001566597,0.0001187681,0.0008190922,0.00001049796,0.00002281398,0.0002490884,0.0000125143,0.005904487,0.9871272,0.0001529057,0.005549429,0.00001749075],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3454074,0.001772519,0.6327612,0.0003877758,0.0001778556,0.0018265,0.007918797,0.004029807,0.005718178],"genre_scores_gemma":[0.5255847,0.002492379,0.4454515,0.0003458719,0.00003955342,0.001494759,0.0148473,0.0005869311,0.009156936],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.00213945,"threshold_uncertainty_score":0.007157147,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03035190781570126,"score_gpt":0.288474008727896,"score_spread":0.2581221009121947,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}