{"id":"W4223594480","doi":"10.1038/s41467-022-29508-w","title":"Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification","year":2022,"lang":"en","type":"article","venue":"Nature Communications","topic":"Photochromic and Fluorescence Chemistry","field":"Materials Science","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"KU Leuven; Fonds Wetenschappelijk Onderzoek; Natural Sciences and Engineering Research Council of Canada; Vlaamse regering; European Commission; Agence Nationale de la Recherche; University of Alberta","keywords":"Biosensor; Photochromism; Fluorophore; Analyte; Fluorescent protein; Fluorescence; Materials science; Nanotechnology; Biological system; Computer science; Chemistry; Optics; Physics; Chromatography; Biochemistry; Biology; Green fluorescent protein","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004779828,0.0001236227,0.0001823842,0.00007586868,0.0004711063,0.00003371902,0.0008084588,0.00009030435,0.00009195371],"category_scores_gemma":[0.00006143055,0.000130641,0.00005594985,0.0001677741,0.0003391785,0.00009698345,0.0006352573,0.00044532,0.000001832355],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002803874,"about_ca_system_score_gemma":0.00008327355,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005382428,"about_ca_topic_score_gemma":0.00001684545,"domain_scores_codex":[0.998812,0.0001476297,0.0002939914,0.000232644,0.0003568278,0.0001569542],"domain_scores_gemma":[0.998347,0.00006734687,0.0002557506,0.001170011,0.0001135703,0.00004631173],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001947054,0.0001600086,0.00006309297,0.00002621096,0.00001113131,5.918407e-7,0.0003988956,0.000021635,0.9986655,0.00023807,0.0001914341,0.0002039847],"study_design_scores_gemma":[0.0001745719,0.00002885048,0.0004357756,0.00004093176,0.00003356421,0.00001496981,0.001097278,0.00197872,0.99458,0.00005186216,0.001424559,0.0001388601],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9951153,0.003543616,0.0001777637,0.0002164233,0.0001675936,0.0003872524,0.0001269516,0.00004000807,0.0002251164],"genre_scores_gemma":[0.9967942,0.00007671065,0.002892921,0.00004296406,0.0000166888,0.00009639432,0.00003998607,0.00001541592,0.00002477933],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004085416,"threshold_uncertainty_score":0.5327384,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05729599602361457,"score_gpt":0.27806138678001,"score_spread":0.2207653907563955,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}