{"id":"W4223897534","doi":"10.3390/pathogens11040462","title":"Comparative Evaluation of Six SARS-CoV-2 Real-Time RT-PCR Diagnostic Approaches Shows Substantial Genomic Variant–Dependent Intra- and Inter-Test Variability, Poor Interchangeability of Cycle Threshold and Complementary Turn-Around Times","year":2022,"lang":"en","type":"article","venue":"Pathogens","topic":"SARS-CoV-2 detection and testing","field":"Medicine","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Javna Agencija za Raziskovalno Dejavnost RS; Univerza v Ljubljani; European Commission; Government of Canada; Indian Council of Medical Research; Association of Public Health Laboratories","keywords":"Computational biology; Biology; Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Real-time polymerase chain reaction; Diagnostic test; Serial dilution; Virology; Genetics; Coronavirus disease 2019 (COVID-19); Medicine; Gene; Pathology","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.003198939,0.0002574404,0.0006758362,0.0001852531,0.0001585326,0.00002654555,0.0001501798,0.00006495828,0.0002498425],"category_scores_gemma":[0.0007841619,0.0002564261,0.00009415425,0.0002310244,0.0003042016,0.00009667174,0.0004346021,0.0002880278,0.000002677215],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000267633,"about_ca_system_score_gemma":0.0001190873,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004324492,"about_ca_topic_score_gemma":0.0003496091,"domain_scores_codex":[0.9971951,0.0006358045,0.000718295,0.000578271,0.0006106115,0.0002619169],"domain_scores_gemma":[0.9974912,0.001517334,0.0003344427,0.0003850433,0.0002114093,0.00006061538],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003171162,0.0007910835,0.1015657,0.0001775002,0.0001789317,0.00001445762,0.00438075,0.00003949804,0.8905962,0.00007381028,0.00003413439,0.001830836],"study_design_scores_gemma":[0.006886697,0.002592476,0.1190986,0.0001932123,0.0019466,0.0006725194,0.006992763,0.218597,0.6387607,0.003571191,0.00004633508,0.0006419249],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9962912,0.0001882596,0.0002246346,0.00009773442,0.0001327733,0.001463688,0.0006368741,0.00004558009,0.0009192508],"genre_scores_gemma":[0.9987775,0.00001003881,0.0007803483,0.0001129486,0.0000727097,0.0001098633,0.000106631,0.00002613567,0.00000379304],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2518355,"threshold_uncertainty_score":0.9999888,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1213394324144304,"score_gpt":0.3278448439308017,"score_spread":0.2065054115163713,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}