{"id":"W4223992660","doi":"10.1186/s12879-022-07356-9","title":"Development and evaluation of a novel fast broad-range PCR and sequencing assay (FBR-PCR/S) using dual priming oligonucleotides targeting the ITS/LSU gene regions for rapid diagnosis of invasive fungal diseases: multi-year experience in a large Canadian healthcare zone","year":2022,"lang":"en","type":"article","venue":"BMC Infectious Diseases","topic":"Antifungal resistance and susceptibility","field":"Medicine","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; Calgary Laboratory Services","funders":"Alberta Precision Laboratories; Calgary Laboratory Services","keywords":"Biology; Internal transcribed spacer; Parasitology; Voriconazole; Medical microbiology; Real-time polymerase chain reaction; Amplicon; Polymerase chain reaction; Internal medicine; Microbiology; Medicine; Gene; Genetics; Antifungal","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002824373,0.0008278823,0.0004151814,0.0008606865,0.0008819557,0.0009324535,0.0008446745,0.0006444037,0.0005935726],"category_scores_gemma":[0.003183248,0.0003081039,0.0003158073,0.0006022445,0.001063278,0.0002982086,0.0005455826,0.0004710509,0.0002528452],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0044058,"about_ca_system_score_gemma":0.007591465,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.4076278,"about_ca_topic_score_gemma":0.5832922,"domain_scores_codex":[0.9969726,0.0004588343,0.0001437276,0.0006207342,0.00152721,0.0002769439],"domain_scores_gemma":[0.9970801,0.0005949018,0.0004777237,0.0001064633,0.001399744,0.0003410044],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"observational","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001253087,0.0005531289,0.6217995,0.0006509227,0.0001342964,0.0008840641,0.002127033,0.002685497,0.2189494,0.0001958226,0.001184889,0.1495823],"study_design_scores_gemma":[0.0002046043,0.006367939,0.8692747,0.0001811243,0.0003512792,0.00555846,0.002351436,0.01649962,0.08714327,0.0000808554,0.01183313,0.0001534869],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9831704,0.002329427,0.0109707,0.000335188,0.00004607657,0.0004649878,0.0005268904,0.0001684185,0.00198787],"genre_scores_gemma":[0.9438465,0.001415644,0.05277538,0.0002877017,0.00003371475,0.00009621133,0.0007388477,0.00002707716,0.0007787747],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.4076278,"threshold_uncertainty_score":0.8105103,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1096758599123823,"score_gpt":0.3355099639535801,"score_spread":0.2258341040411979,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}