{"id":"W4224211986","doi":"10.1186/s13071-022-05236-4","title":"Stable transformation of fluorescent proteins into Nosema bombycis by electroporation","year":2022,"lang":"en","type":"article","venue":"Parasites & Vectors","topic":"Parasitic Infections and Diagnostics","field":"Immunology and Microbiology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ministry of Agriculture","funders":"Natural Science Foundation of Chongqing; National Natural Science Foundation of China","keywords":"Microsporidia; Biology; Transformation (genetics); Electroporation; Plasmid; Gene; Intracellular parasite; Transformation efficiency; Green fluorescent protein; Transduction (biophysics); Microbiology; Cell biology; Genetics; Spore; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002265501,0.0003921126,0.0002416598,0.0002564603,0.0001648325,0.0002147108,0.0002853737,0.0002643971,0.0006013723],"category_scores_gemma":[0.0001442108,0.0001412553,0.0002780267,0.0002267677,0.0002331832,0.0001648287,0.0003450809,0.0006238029,0.0003303624],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004125912,"about_ca_system_score_gemma":0.0002330543,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009248449,"about_ca_topic_score_gemma":0.001205595,"domain_scores_codex":[0.9998286,0.00002248202,0.00002025529,0.00005263181,0.00005154181,0.00002448452],"domain_scores_gemma":[0.9998956,0.00002231383,0.00003465658,0.00001764489,0.00001331114,0.00001637761],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000163827,0.000006376221,0.00006842719,0.00001576838,0.000001984133,0.00002100878,0.00001073604,0.00001594167,0.9991836,0.00005637768,0.00001111211,0.000592204],"study_design_scores_gemma":[0.00001104049,0.0001280826,0.002976744,0.000007548676,0.00001904258,0.0002275996,0.0000232577,0.001017208,0.9907444,0.00005091104,0.004788083,0.000006116253],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9445286,0.001621603,0.04972045,0.0001193891,0.00008571224,0.0002463923,0.001174697,0.0002910896,0.002212072],"genre_scores_gemma":[0.9411831,0.001592896,0.04756118,0.00006155264,0.00001505399,0.0002013133,0.00303387,0.0001306829,0.006220318],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009248449,"threshold_uncertainty_score":0.002993643,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005208702661379914,"score_gpt":0.230749525145232,"score_spread":0.2255408224838521,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}