{"id":"W4224211986","doi":"10.1186/s13071-022-05236-4","title":"Stable transformation of fluorescent proteins into Nosema bombycis by electroporation","year":2022,"lang":"en","type":"article","venue":"Parasites & Vectors","topic":"Parasitic Infections and Diagnostics","field":"Immunology and Microbiology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ministry of Agriculture","funders":"Natural Science Foundation of Chongqing; National Natural Science Foundation of China","keywords":"Microsporidia; Biology; Transformation (genetics); Electroporation; Plasmid; Gene; Intracellular parasite; Transformation efficiency; Green fluorescent protein; Transduction (biophysics); Microbiology; Cell biology; Genetics; Spore; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.0002068613,0.0001280569,0.0001926646,0.0001261828,0.000419432,0.00001029439,0.0001100237,0.00007433142,0.001239958],"category_scores_gemma":[0.00006151071,0.0001303238,0.00007023713,0.0002899979,0.00007010662,0.0001168467,0.00002508597,0.0002122497,0.0001034918],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001509074,"about_ca_system_score_gemma":0.00007372112,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000952699,"about_ca_topic_score_gemma":0.00005330586,"domain_scores_codex":[0.9990197,0.0001749815,0.0003116493,0.0001681526,0.00007004171,0.0002554067],"domain_scores_gemma":[0.9995294,0.00008737832,0.0001359303,0.0001616874,0.00006698193,0.00001862168],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008149059,0.0005064983,0.02492382,0.00003051805,0.00009493485,0.000001182312,0.001691305,0.0003271373,0.9554744,0.002333259,0.01265689,0.001878539],"study_design_scores_gemma":[0.0006982019,0.000734701,0.007604496,0.000006227636,0.00005931248,0.00002399506,0.00034691,0.0000751009,0.9439222,0.0002968495,0.04603031,0.0002016865],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9922729,0.001199853,0.00441997,0.0001230615,0.0002558942,0.0004908172,0.0001068736,0.00006247882,0.001068188],"genre_scores_gemma":[0.9981544,0.00004298065,0.00008259548,0.00004879961,0.00001185743,0.0001629434,0.00125476,0.00001222844,0.0002294405],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03337341,"threshold_uncertainty_score":0.9996731,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005208702661379914,"score_gpt":0.230749525145232,"score_spread":0.2255408224838521,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}