{"id":"W4225426339","doi":"10.1016/j.scr.2022.102806","title":"Generation of homozygous PRKN, PINK1 and double PINK1/PRKN knockout cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing","year":2022,"lang":"en","type":"article","venue":"Stem Cell Research","topic":"Genetics and Neurodevelopmental Disorders","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Montreal Neurological Institute and Hospital","funders":"Canadian Institutes of Health Research; McGill University; Québec Consortium for Drug Discovery; Michael J. Fox Foundation for Parkinson's Research","keywords":"PINK1; Biology; Induced pluripotent stem cell; CRISPR; Ubiquitin ligase; Cell biology; Genetics; Molecular biology; Embryonic stem cell; Mitophagy; Ubiquitin; Autophagy; Gene; Apoptosis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003641783,0.0004593418,0.0003984825,0.0005127278,0.0003036978,0.0004425326,0.0005626297,0.0005766153,0.003022985],"category_scores_gemma":[0.000225212,0.0003105315,0.0004179928,0.0003205888,0.0003988839,0.000241182,0.0005261014,0.0008383959,0.001379191],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000258813,"about_ca_system_score_gemma":0.0003302238,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007447483,"about_ca_topic_score_gemma":0.001403442,"domain_scores_codex":[0.9996303,0.00001982219,0.00006818432,0.00009979765,0.0001375159,0.00004433966],"domain_scores_gemma":[0.999762,0.00003364356,0.00005884037,0.00004652797,0.00003337416,0.00006562108],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001051184,0.00004618114,0.000341081,0.00006051599,0.00001504703,0.0003325128,0.00006879539,0.0002411906,0.9948727,0.0006751611,0.00030574,0.002935945],"study_design_scores_gemma":[0.00006988522,0.000186132,0.003541199,0.00001715642,0.00006046032,0.001772012,0.00005365557,0.002290243,0.9809825,0.0002168385,0.01078564,0.00002416712],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9052315,0.001044053,0.07279959,0.0002505409,0.0002731401,0.001061796,0.01081648,0.001186269,0.007336746],"genre_scores_gemma":[0.9271713,0.001133829,0.03830445,0.0001852945,0.0000226074,0.000975311,0.009775272,0.0004587045,0.02197304],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003022985,"threshold_uncertainty_score":0.01011294,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1344146473029513,"score_gpt":0.3411191464145473,"score_spread":0.206704499111596,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}