{"id":"W4225426339","doi":"10.1016/j.scr.2022.102806","title":"Generation of homozygous PRKN, PINK1 and double PINK1/PRKN knockout cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing","year":2022,"lang":"en","type":"article","venue":"Stem Cell Research","topic":"Genetics and Neurodevelopmental Disorders","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Montreal Neurological Institute and Hospital","funders":"Canadian Institutes of Health Research; McGill University; Québec Consortium for Drug Discovery; Michael J. Fox Foundation for Parkinson's Research","keywords":"PINK1; Biology; Induced pluripotent stem cell; CRISPR; Ubiquitin ligase; Cell biology; Genetics; Molecular biology; Embryonic stem cell; Mitophagy; Ubiquitin; Autophagy; Gene; Apoptosis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006036872,0.0002145469,0.0002349225,0.0001656301,0.0005811937,0.00006876851,0.0003109342,0.0001328355,0.00003490263],"category_scores_gemma":[0.000003906566,0.0002331848,0.00006348075,0.0002688204,0.00007904949,0.000007160063,0.0008446816,0.0003795269,0.000005239136],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00007042092,"about_ca_system_score_gemma":0.000360589,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005516725,"about_ca_topic_score_gemma":0.00009722712,"domain_scores_codex":[0.9972717,0.0003581217,0.0004454495,0.0007029902,0.0006680804,0.0005536797],"domain_scores_gemma":[0.9989887,0.00003506929,0.0001656367,0.0004003168,0.0002364417,0.0001738013],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001620788,0.0001637633,0.0003296488,0.0001233335,0.00001803302,0.0000106595,0.0003532614,0.0008412814,0.9946445,0.000002441517,0.000979726,0.002371259],"study_design_scores_gemma":[0.001207451,0.0008793804,0.0002192243,0.00001231329,0.00001152299,0.000006095851,0.002550322,0.001554827,0.9886746,0.000008822924,0.004649984,0.0002254299],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9949868,0.003183144,0.0001126099,0.0001222726,0.0004302163,0.0006526476,0.00008656025,0.000009408142,0.0004163556],"genre_scores_gemma":[0.9975648,0.0006724605,0.0004034939,0.00009750883,0.0001839646,0.00006280383,0.0001258106,0.00005414485,0.0008350493],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005969886,"threshold_uncertainty_score":0.9509,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1344146473029513,"score_gpt":0.3411191464145473,"score_spread":0.206704499111596,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}