{"id":"W4226067321","doi":"10.1038/s41598-021-02986-6","title":"CRISPR/Cas9-mediated base-editing enables a chain reaction through sequential repair of sgRNA scaffold mutations","year":2021,"lang":"en","type":"article","venue":"Scientific Reports","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Institute of Medical Science, University of Tokyo; Japan Society for the Promotion of Science","keywords":"CRISPR; Cas9; Subgenomic mRNA; Biology; Genome editing; Computational biology; Chain reaction; Gene; Genetics; Cell biology; Chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004400472,0.0005142145,0.0004029822,0.0002186271,0.0001872878,0.0005793822,0.0004753228,0.0004946233,0.0009874146],"category_scores_gemma":[0.0004192153,0.0002439214,0.0004361207,0.0001806038,0.0004767556,0.0003733973,0.0004218489,0.0009267976,0.0005608145],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003607413,"about_ca_system_score_gemma":0.0003808204,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000417855,"about_ca_topic_score_gemma":0.0006849765,"domain_scores_codex":[0.9995496,0.00006159936,0.00005068171,0.0001651679,0.000120064,0.00005288385],"domain_scores_gemma":[0.9995851,0.00009743923,0.0001336813,0.00009471409,0.00004036516,0.00004867283],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003119585,0.00001410379,0.00009873815,0.0000425749,0.000007190505,0.00004644525,0.00001351095,0.0002417771,0.9961665,0.0004725857,0.00004820905,0.002816991],"study_design_scores_gemma":[0.000003276796,0.0000627527,0.0001423993,0.000001771419,0.000007105874,0.00007390776,0.000004342764,0.001116267,0.9961895,0.00007461861,0.00231856,0.00000534583],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6184015,0.001875065,0.3705851,0.0002704836,0.000244141,0.0003683917,0.0005965026,0.002653423,0.005005446],"genre_scores_gemma":[0.9044725,0.0009410519,0.08964518,0.00007981758,0.00002048956,0.0001118799,0.0005676068,0.0001746705,0.003986853],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009874146,"threshold_uncertainty_score":0.00330323,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01363175900259216,"score_gpt":0.2897770865377464,"score_spread":0.2761453275351543,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}