{"id":"W4229009276","doi":"10.1002/bit.28129","title":"High‐level production of wild‐type and oxidation‐resistant recombinant alpha‐1‐antitrypsin in glycoengineered CHO cells","year":2022,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Université de Montréal; National Research Council Canada","funders":"","keywords":"Glycosylation; Fucosylation; Chinese hamster ovary cell; Recombinant DNA; Neutrophil elastase; Cell culture; Proteases; Glycan; Molecular biology; Biology; Biochemistry; Chemistry; Gene; Glycoprotein; Enzyme; Immunology; Inflammation","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001096704,0.0001211937,0.0001407877,0.0001629344,0.00008668924,0.000006264617,0.00008290452,0.0001468144,0.000009150453],"category_scores_gemma":[0.000040695,0.000123411,0.00002141886,0.0002639455,0.00006746752,0.000005526869,0.0001745916,0.000135365,3.223658e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001486445,"about_ca_system_score_gemma":0.0000218902,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004480858,"about_ca_topic_score_gemma":0.0000140017,"domain_scores_codex":[0.9992853,0.00002130408,0.000183044,0.0002874398,0.00006070358,0.0001622056],"domain_scores_gemma":[0.9996933,0.0000047507,0.00005745283,0.0001866183,0.00002604425,0.00003186159],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001030712,0.0000620148,0.0006456038,0.00004337891,0.00002535861,0.000003177908,0.00001254377,0.001610992,0.9954582,0.000346273,0.0004902519,0.001199109],"study_design_scores_gemma":[0.0004782985,0.0005204886,0.004073029,0.00002300687,0.0000137231,0.00003181306,0.00007131861,0.0003700105,0.987236,0.00007598827,0.006923022,0.0001833044],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9964321,0.002014356,0.0003298773,0.0002985642,0.0006862646,0.0001847221,0.00001916391,0.00003023242,0.000004674267],"genre_scores_gemma":[0.9980007,0.0009879142,0.0007729909,0.00002029262,0.00003393897,0.00003195155,0.00004087176,0.00001326878,0.00009808774],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008222228,"threshold_uncertainty_score":0.5032556,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00784788658317084,"score_gpt":0.2069762817878312,"score_spread":0.1991283952046604,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}