{"id":"W4229009276","doi":"10.1002/bit.28129","title":"High‐level production of wild‐type and oxidation‐resistant recombinant alpha‐1‐antitrypsin in glycoengineered CHO cells","year":2022,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Université de Montréal; National Research Council Canada","funders":"","keywords":"Glycosylation; Fucosylation; Chinese hamster ovary cell; Recombinant DNA; Neutrophil elastase; Cell culture; Proteases; Glycan; Molecular biology; Biology; Biochemistry; Chemistry; Gene; Glycoprotein; Enzyme; Immunology; Inflammation","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008560838,0.0009470089,0.0007857258,0.0004119199,0.0003214154,0.0009647053,0.0009697469,0.0007977362,0.001117058],"category_scores_gemma":[0.0005089784,0.0003601692,0.0008312157,0.0007094688,0.0004428416,0.0004730235,0.0008428993,0.001607453,0.001822273],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007872724,"about_ca_system_score_gemma":0.0003858082,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001263437,"about_ca_topic_score_gemma":0.001174786,"domain_scores_codex":[0.999324,0.00009052071,0.0001304492,0.0001564795,0.0002059761,0.000092629],"domain_scores_gemma":[0.9996306,0.00005958396,0.0000718199,0.0001083783,0.00007457512,0.0000550469],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006850484,0.00003725012,0.00007834615,0.00007900521,0.00001366885,0.00007486801,0.00004844502,0.0001212658,0.9982703,0.0002130557,0.0001069555,0.0008883054],"study_design_scores_gemma":[0.00003715414,0.000188043,0.0007403821,0.00001352174,0.00003724287,0.0002356193,0.0000334135,0.001390828,0.9877507,0.00008791051,0.009466674,0.00001850229],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9004803,0.003376777,0.07728579,0.0005632869,0.000521328,0.0005943334,0.009335337,0.00128826,0.006554526],"genre_scores_gemma":[0.8852007,0.002855585,0.07371508,0.0002482613,0.00007505667,0.0007171485,0.01913483,0.0007361554,0.01731714],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001263437,"threshold_uncertainty_score":0.005712092,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00784788658317084,"score_gpt":0.2069762817878312,"score_spread":0.1991283952046604,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}