{"id":"W4229032942","doi":"10.3389/fbioe.2022.871933","title":"FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult Recombinant Proteins in Drosophila S2 Cells","year":2022,"lang":"en","type":"article","venue":"Frontiers in Bioengineering and Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Ministero dello Sviluppo Economico; Fundação de Amparo à Pesquisa do Estado de São Paulo; Ontario Ministry of Economic Development and Innovation; European Federation of Pharmaceutical Industries and Associations; Novartis Pharma; Wellcome Trust; Kennedy Trust for Rheumatology Research; Genome Canada; University of Tokyo; Pfizer","keywords":"Sf9; Schneider 2 cells; Biology; Drosophila melanogaster; Cell biology; Bottleneck; Recombinant DNA; Computational biology; Spodoptera; Throughput; Glycoprotein; Cell culture; clone (Java method); Gene; Genetics; RNA interference; Computer science","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001130683,0.0001797265,0.0002598991,0.0002793876,0.00008053559,0.00000807895,0.0001901439,0.0002776881,0.000006198337],"category_scores_gemma":[0.00002978753,0.0001756818,0.0000497475,0.0003342078,0.00009490691,0.000005138695,0.0003221814,0.0002830262,2.140192e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003990382,"about_ca_system_score_gemma":0.00003011963,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000513661,"about_ca_topic_score_gemma":0.00000867699,"domain_scores_codex":[0.9988665,0.00005597668,0.0002876198,0.0004100807,0.00009439848,0.0002854029],"domain_scores_gemma":[0.9995732,0.000004318797,0.00008362906,0.0002776861,0.00001810332,0.00004300292],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001488595,0.0001490349,0.002439253,0.00005235803,0.00001255274,0.00001308699,0.00006354933,0.009284582,0.9852555,0.00004198195,0.001631455,0.0009078179],"study_design_scores_gemma":[0.001109487,0.0007032108,0.0006830814,0.00005805149,0.000005295421,0.00001714523,0.0003341292,0.005188312,0.9865456,0.0003177699,0.004750509,0.0002873811],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9915855,0.002488865,0.004688053,0.0001750757,0.0006597223,0.0003054883,0.00003548909,0.00004108829,0.00002070156],"genre_scores_gemma":[0.9960995,0.0009190955,0.002662231,0.00003453574,0.00002442407,0.000125456,0.00004580131,0.00002255885,0.00006636979],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004514012,"threshold_uncertainty_score":0.7164095,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00422732072570059,"score_gpt":0.1954347756871808,"score_spread":0.1912074549614803,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}