{"id":"W4229032942","doi":"10.3389/fbioe.2022.871933","title":"FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult Recombinant Proteins in Drosophila S2 Cells","year":2022,"lang":"en","type":"article","venue":"Frontiers in Bioengineering and Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Ministero dello Sviluppo Economico; Fundação de Amparo à Pesquisa do Estado de São Paulo; Ontario Ministry of Economic Development and Innovation; European Federation of Pharmaceutical Industries and Associations; Novartis Pharma; Wellcome Trust; Kennedy Trust for Rheumatology Research; Genome Canada; University of Tokyo; Pfizer","keywords":"Sf9; Schneider 2 cells; Biology; Drosophila melanogaster; Cell biology; Bottleneck; Recombinant DNA; Computational biology; Spodoptera; Throughput; Glycoprotein; Cell culture; clone (Java method); Gene; Genetics; RNA interference; Computer science","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006157092,0.0007483795,0.0006568137,0.0004469112,0.0003889342,0.0008882123,0.0006025471,0.0004730074,0.001899397],"category_scores_gemma":[0.0002991904,0.0003987118,0.0006249368,0.0004194397,0.0003227545,0.0003807075,0.0007404894,0.001201094,0.002685374],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00076539,"about_ca_system_score_gemma":0.0005375881,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001509157,"about_ca_topic_score_gemma":0.003021338,"domain_scores_codex":[0.9996064,0.00004889281,0.00004584717,0.00008491156,0.0001646853,0.00004925551],"domain_scores_gemma":[0.999833,0.00003000285,0.00003160253,0.00003509874,0.00003435274,0.00003601097],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006234109,0.00002661876,0.0001258376,0.00007991018,0.000006782725,0.00004823943,0.00003856494,0.0001664966,0.9956542,0.0002452482,0.0004200848,0.00312579],"study_design_scores_gemma":[0.00002166399,0.0001445513,0.0006604771,0.00001451721,0.00001364748,0.0001912835,0.00002439781,0.00191696,0.9785894,0.00007524192,0.01833234,0.00001555958],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7080895,0.003117912,0.2549476,0.0006110487,0.0002726731,0.001746612,0.0115601,0.006069984,0.01358458],"genre_scores_gemma":[0.6414887,0.003729348,0.2947924,0.0003388048,0.00004972845,0.001194619,0.02685584,0.001521407,0.03002909],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001899397,"threshold_uncertainty_score":0.006354094,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00422732072570059,"score_gpt":0.1954347756871808,"score_spread":0.1912074549614803,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}