{"id":"W4229056060","doi":"10.1002/bit.28127","title":"Production of afucosylated antibodies in CHO cells by coexpression of an anti‐FUT8 intrabody","year":2022,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada","funders":"","keywords":"Fucosylation; Antibody-dependent cell-mediated cytotoxicity; Fucosyltransferase; Fucose; Glycan; Antibody; Brefeldin A; Effector; Chemistry; Golgi apparatus; Western blot; Fragment crystallizable region; Chinese hamster ovary cell; Biochemistry; Glycosylation; Molecular biology; Cytotoxicity; Cell biology; Endoplasmic reticulum; Biology; Glycoprotein; In vitro; Enzyme; Immunology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003287585,0.0007374117,0.0005082679,0.0003035352,0.0002154269,0.0005183625,0.000609546,0.000580716,0.0007817437],"category_scores_gemma":[0.0001881387,0.0002548827,0.0005581554,0.0005012412,0.0002679241,0.0003733501,0.000637299,0.001075349,0.0009286007],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005911773,"about_ca_system_score_gemma":0.0002771016,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001184336,"about_ca_topic_score_gemma":0.001084005,"domain_scores_codex":[0.9997309,0.00004032011,0.00004258579,0.00004936699,0.00008569191,0.00005114083],"domain_scores_gemma":[0.9998738,0.00001706518,0.00002807258,0.00003754958,0.00002515522,0.00001830314],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000352278,0.00001457721,0.00004680114,0.00002599022,0.000006375662,0.0000330739,0.00001891917,0.00004757956,0.9990227,0.00009754475,0.00002036726,0.0006307787],"study_design_scores_gemma":[0.00001235853,0.0001165359,0.0003374224,0.000003657984,0.00001659724,0.0001003322,0.000008291253,0.0005016407,0.9966567,0.00002363227,0.002218468,0.000004371137],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9559958,0.001996479,0.03499486,0.0002860152,0.0001857182,0.0002486398,0.001475051,0.0003666205,0.004450786],"genre_scores_gemma":[0.9532301,0.001973315,0.03207908,0.0001395411,0.00005027578,0.0002604814,0.003002794,0.0001659733,0.00909859],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001184336,"threshold_uncertainty_score":0.004289269,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008742786334760878,"score_gpt":0.2542128026987178,"score_spread":0.2454700163639569,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}