{"id":"W4229056060","doi":"10.1002/bit.28127","title":"Production of afucosylated antibodies in CHO cells by coexpression of an anti‐FUT8 intrabody","year":2022,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada","funders":"","keywords":"Fucosylation; Antibody-dependent cell-mediated cytotoxicity; Fucosyltransferase; Fucose; Glycan; Antibody; Brefeldin A; Effector; Chemistry; Golgi apparatus; Western blot; Fragment crystallizable region; Chinese hamster ovary cell; Biochemistry; Glycosylation; Molecular biology; Cytotoxicity; Cell biology; Endoplasmic reticulum; Biology; Glycoprotein; In vitro; Enzyme; Immunology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001732425,0.00009319302,0.00025354,0.0003163024,0.00004774965,0.000001561974,0.00008223082,0.0001223677,0.00003059987],"category_scores_gemma":[0.00002054085,0.00007976309,0.00002419505,0.0003381186,0.0001584499,0.0000388599,0.000120733,0.0003223432,3.383985e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001613117,"about_ca_system_score_gemma":0.00001637701,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007354032,"about_ca_topic_score_gemma":0.00000252855,"domain_scores_codex":[0.9992586,0.00002272501,0.0002156646,0.0001936029,0.0001367897,0.0001725818],"domain_scores_gemma":[0.9997346,0.00001439576,0.00005155062,0.0001411371,0.00002424707,0.00003400371],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000161574,0.0001765246,0.005274447,0.0001895909,0.00002564856,0.0000140657,0.00007740263,0.0001584945,0.9877847,0.00013769,0.00007702034,0.005922851],"study_design_scores_gemma":[0.0003500907,0.0004673967,0.00799309,0.00006543406,0.00001075564,0.00005557006,0.0001784473,0.002307616,0.9853405,0.00001520927,0.003138016,0.00007788317],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9971797,0.001423292,0.00003106584,0.0009297342,0.0001164083,0.0002242045,0.00002980318,0.00004333544,0.0000224739],"genre_scores_gemma":[0.9983997,0.0009674476,0.0004528958,0.00000807499,0.00001662537,0.000009871227,0.00003434931,0.0000084348,0.0001026508],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005844967,"threshold_uncertainty_score":0.3252644,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008742786334760878,"score_gpt":0.2542128026987178,"score_spread":0.2454700163639569,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}