{"id":"W4231027630","doi":"10.1139/cjm-46-5-474","title":"Overproduction in <i>Escherichia coli </i>of the pectin methylesterase A from <i>Erwinia chrysanthemi</i> 3937: One-step purification, biochemical characterization, and production of polyclonal antibodies","year":2000,"lang":"en","type":"article","venue":"Canadian Journal of Microbiology","topic":"Biofuel production and bioconversion","field":"Engineering","cited_by":2,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"","keywords":"Escherichia coli; Overproduction; Pectin; Pectin lyase; Pectinesterase; Enterobacteriaceae; Erwinia; Chemistry; Biology; Microbiology; Biochemistry; Pectinase; Enzyme; Gene","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003668138,0.001294689,0.0007648996,0.0004405832,0.0002248602,0.0006290597,0.0008479947,0.0004500336,0.001087981],"category_scores_gemma":[0.0002615382,0.000418838,0.000532052,0.0009288613,0.0003259814,0.0003355267,0.0004603397,0.001349017,0.001662539],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001209562,"about_ca_system_score_gemma":0.0006738369,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.006600747,"about_ca_topic_score_gemma":0.004990656,"domain_scores_codex":[0.9994549,0.00007210909,0.00007504827,0.0001140719,0.0001789102,0.0001049184],"domain_scores_gemma":[0.9997103,0.00005976833,0.00006484821,0.00007198458,0.00003466298,0.00005848572],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007106431,0.00007307287,0.0001262083,0.00005939004,0.00001458596,0.00004052947,0.00002345838,0.00009895561,0.9980549,0.0001558374,0.0002036862,0.001078351],"study_design_scores_gemma":[0.00001957157,0.0001039672,0.001275608,0.000007150927,0.00002331523,0.0001293222,0.00001719195,0.000660508,0.9939568,0.00004307152,0.003755468,0.000007985953],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9492567,0.002253506,0.02811587,0.0008271603,0.0003367334,0.0003655888,0.01075896,0.001341711,0.006743767],"genre_scores_gemma":[0.918974,0.001527652,0.02652679,0.000315961,0.00005474815,0.0002748221,0.03120879,0.0004595463,0.02065772],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006600747,"threshold_uncertainty_score":0.01312464,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00716151487383992,"score_gpt":0.1858470278751804,"score_spread":0.1786855130013404,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}