{"id":"W4233024833","doi":"10.1371/journal.pone.0121043","title":"tRNAGlu Increases the Affinity of Glutamyl-tRNA Synthetase for Its Inhibitor Glutamyl-Sulfamoyl-Adenosine, an Analogue of the Aminoacylation Reaction Intermediate Glutamyl-AMP: Mechanistic and Evolutionary Implications","year":2015,"lang":"en","type":"article","venue":"PLoS ONE","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval; Concordia University; PROTEO","funders":"Fonds de recherche du Québec – Nature et technologies; Natural Sciences and Engineering Research Council of Canada; Université de Sherbrooke","keywords":"Aminoacylation; Aminoacyl tRNA synthetase; Transfer RNA; Biochemistry; Biology; Adenosine; Chemistry; Stereochemistry; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000113039,0.0003721213,0.0003591763,0.0001147364,0.000103058,0.0003465305,0.0002505522,0.0004460321,0.004327531],"category_scores_gemma":[0.0002628551,0.0001451446,0.0002213581,0.00008486007,0.0001868661,0.0001418058,0.0002073413,0.0003566427,0.0006515541],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002076244,"about_ca_system_score_gemma":0.0001536804,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000381381,"about_ca_topic_score_gemma":0.0003304793,"domain_scores_codex":[0.9998643,0.0000332551,0.0000106468,0.00003114074,0.00002998434,0.00003056299],"domain_scores_gemma":[0.9998343,0.00003925277,0.00004717386,0.00001631817,0.00001197742,0.0000509892],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000320058,0.00003573931,0.0001780347,0.00003446025,0.00000673889,0.00004696065,0.000009551642,0.00006180519,0.9982024,0.00005426443,0.00003887832,0.001010935],"study_design_scores_gemma":[0.00002752022,0.0004918157,0.003842102,0.000006011964,0.00001106446,0.0001791446,0.00002662815,0.0008156073,0.9927992,0.00005602893,0.001736991,0.000007994419],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9972981,0.0007582789,0.0007019953,0.0001036979,0.00004688894,0.000006710073,0.00008734647,0.00008627371,0.0009108997],"genre_scores_gemma":[0.9972454,0.0002385628,0.0005989792,0.00004795858,0.00001083344,0.000008787017,0.00008591808,0.00003043771,0.001733157],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004327531,"threshold_uncertainty_score":0.01447707,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0636332126425648,"score_gpt":0.2555618991490695,"score_spread":0.1919286865065047,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}