{"id":"W4234556469","doi":"10.1093/nar/gky976","title":"Active site residue identity regulates cleavage preference of LAGLIDADG homing endonucleases","year":2018,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba; Western University","funders":"Argonne National Laboratory; Natural Sciences and Engineering Research Council of Canada; Industrial Macromolecular Crystallography Association Collaborative Access Team; Canadian Institutes of Health Research; Office of Science; U.S. Department of Energy","keywords":"Homing endonuclease; Biology; Active site; Cleavage (geology); DNA; Cleavage factor; Genome; Genetics; Cleavage and polyadenylation specificity factor; Reprogramming; Scissile bond; Endonuclease; Biochemistry; Computational biology; Enzyme; Polyadenylation; Gene; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004611199,0.0003521197,0.000393488,0.0002601817,0.0001282947,0.0007685763,0.0002969911,0.0004386209,0.00124703],"category_scores_gemma":[0.0008032159,0.0002370013,0.0001977806,0.0002119377,0.0003064987,0.0002637023,0.0003562633,0.000686175,0.0007371362],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000313348,"about_ca_system_score_gemma":0.0001968257,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004696889,"about_ca_topic_score_gemma":0.0007695496,"domain_scores_codex":[0.9994863,0.00007841982,0.00005364608,0.000112899,0.0001547836,0.0001139371],"domain_scores_gemma":[0.9994696,0.0001604807,0.000126182,0.00004593332,0.00009210758,0.0001057214],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009068308,0.00002101464,0.001307044,0.00001480668,0.000006505383,0.00003860523,0.00001598589,0.0001842088,0.9971033,0.0000523317,0.00002451358,0.001141009],"study_design_scores_gemma":[0.00001191753,0.0001540239,0.01472718,0.000008385111,0.00002385543,0.0003277559,0.00007412849,0.003808093,0.9796578,0.0001006139,0.001089459,0.00001683856],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9967356,0.0002603555,0.002092633,0.0000236033,0.00000721145,0.0000109198,0.000117817,0.00003579959,0.0007159655],"genre_scores_gemma":[0.9975953,0.0001111188,0.001478982,0.00002869993,0.000001517379,0.000008009982,0.000221278,0.00003964861,0.0005155819],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00124703,"threshold_uncertainty_score":0.004171669,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03837344545888637,"score_gpt":0.3720029924082638,"score_spread":0.3336295469493774,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}