{"id":"W4234702985","doi":"10.1002/ange.201408015","title":"Coumarin‐Based Fluorogenic Probes for No‐Wash Protein Labeling","year":2014,"lang":"en","type":"article","venue":"Angewandte Chemie","topic":"Click Chemistry and Applications","field":"Chemistry","cited_by":69,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"","keywords":"Maleimide; Fluorophore; Chemistry; Reactivity (psychology); Thiol; Fluorescence; Peptide; Reagent; Combinatorial chemistry; Coumarin; Intracellular; Dithiol; Glutathione; Bioorthogonal chemistry; Cysteine; Biochemistry; Organic chemistry; Click chemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004320675,0.0004819829,0.0002591079,0.000315142,0.0002617071,0.0003003141,0.0004781229,0.0004292594,0.001097862],"category_scores_gemma":[0.0003154128,0.0001839171,0.0001854542,0.0003288193,0.0003295715,0.0003822035,0.0002234651,0.0007719907,0.0005497442],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009295142,"about_ca_system_score_gemma":0.0002774828,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006241198,"about_ca_topic_score_gemma":0.001368614,"domain_scores_codex":[0.9997472,0.00004621375,0.00001019912,0.00005492328,0.0000838662,0.00005743386],"domain_scores_gemma":[0.9998253,0.00004302163,0.0000402058,0.00002995816,0.00003460137,0.0000270772],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003654693,0.00001618418,0.00004700929,0.00003705678,0.00000264317,0.00002586783,0.00001234134,0.0001541046,0.9972011,0.000796121,0.0001177906,0.001553325],"study_design_scores_gemma":[0.000003317053,0.00002747862,0.0001638107,0.000001657538,0.000002555305,0.0000405372,0.000002791968,0.0007414835,0.9957419,0.00005125048,0.00322002,0.00000302222],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7476454,0.004989719,0.2380957,0.0006659325,0.0001822725,0.0002424874,0.000683494,0.0008697458,0.006625217],"genre_scores_gemma":[0.9412053,0.002726143,0.04783824,0.0001173128,0.00003481765,0.0001617613,0.0006422009,0.00006915112,0.007205093],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001097862,"threshold_uncertainty_score":0.006744146,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01796576950074885,"score_gpt":0.2495506775268012,"score_spread":0.2315849080260523,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}