{"id":"W4240892966","doi":"10.17504/protocols.io.x45fqy6","title":"Transfection of constructs in diplonemids to enhance the HR pathway, using RS-1 (3-(benzylamino) sulfonyl)-4-bromo-N-(4-bromophenyl) benzamide). v3","year":2019,"lang":"en","type":"preprint","venue":"","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canadian Institute for Advanced Research","funders":"","keywords":"Benzamide; RAD51; Sulfonyl; Transfection; Chemistry; Homologous recombination; HEK 293 cells; Stereochemistry; Molecular biology; Biology; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000246112,0.0006848393,0.0002934822,0.000348832,0.0002647235,0.0004600962,0.000452714,0.0004272478,0.004681729],"category_scores_gemma":[0.0001491803,0.0002900593,0.0002900837,0.0002746989,0.0003043248,0.0002113529,0.0004463411,0.001023796,0.001797474],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006321104,"about_ca_system_score_gemma":0.0003236984,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001655194,"about_ca_topic_score_gemma":0.002197512,"domain_scores_codex":[0.9998104,0.00002527747,0.00002235372,0.00005126146,0.00004683689,0.00004387377],"domain_scores_gemma":[0.9998945,0.00002773875,0.00002427367,0.00001861959,0.000008073067,0.00002665316],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001033706,0.00002773781,0.00009284153,0.00006035826,0.000005899918,0.00005259879,0.00004545352,0.00004950665,0.9973344,0.0007655367,0.0001784417,0.001283967],"study_design_scores_gemma":[0.0000465317,0.00009031182,0.001488186,0.00001078052,0.00001762189,0.0001415148,0.00002677634,0.0006285855,0.9814333,0.00007688194,0.01603233,0.000007065327],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9385108,0.001132755,0.03347753,0.0002744461,0.0003820812,0.0005910953,0.0076415,0.001085104,0.01690466],"genre_scores_gemma":[0.903593,0.000996946,0.02534181,0.000130288,0.0000405319,0.0004823766,0.01858891,0.0005500599,0.05027602],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004681729,"threshold_uncertainty_score":0.01566195,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01390360679085721,"score_gpt":0.3027048152537927,"score_spread":0.2888012084629354,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}