{"id":"W4241425183","doi":"10.1139/w00-044","title":"Molecular cloning, heterologous expression, and functional characterisation of a malate synthase gene from<i>Streptomyces coelicolor</i>A3(2)","year":2000,"lang":"en","type":"article","venue":"Canadian Journal of Microbiology","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National University of Singapore","keywords":"Streptomyces coelicolor; Biology; Streptomyces clavuligerus; Malate synthase; Gene; Malate dehydrogenase; Biochemistry; Glyoxylate cycle; Heterologous expression; ATP synthase; Molecular biology; Molecular cloning; Genetics; Recombinant DNA; Peptide sequence; Streptomyces; Enzyme; Actinomycetales; Bacteria; Mutant","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001866622,0.0006050899,0.0003845745,0.0002953269,0.0002269916,0.0004167064,0.0002601721,0.0002888291,0.0007301631],"category_scores_gemma":[0.0002200795,0.0001595643,0.0005647751,0.0006373774,0.000289003,0.0001657153,0.0002653401,0.0006364447,0.0007373579],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005682115,"about_ca_system_score_gemma":0.0003892846,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003157534,"about_ca_topic_score_gemma":0.002156507,"domain_scores_codex":[0.9998446,0.00002834541,0.0000196827,0.00003404616,0.0000388511,0.0000345363],"domain_scores_gemma":[0.9998252,0.00003564554,0.00003849834,0.00001760778,0.00002487992,0.00005823479],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006857097,0.00002156258,0.0002152319,0.00003352723,0.000002998146,0.00005302334,0.00001730719,0.00008659021,0.9987168,0.00005930951,0.00002323826,0.0007019468],"study_design_scores_gemma":[0.00005480287,0.0003350705,0.01886623,0.00001591445,0.0000389763,0.00087169,0.0001002477,0.003201793,0.9695104,0.0001206644,0.006865262,0.00001897853],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9793528,0.0007636332,0.0134759,0.0001816216,0.00003611092,0.0001351962,0.00420886,0.0001781362,0.001667912],"genre_scores_gemma":[0.9551929,0.0008631871,0.02758336,0.00008124106,0.00002238467,0.0001251147,0.01261369,0.0001150454,0.003403064],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003157534,"threshold_uncertainty_score":0.006278276,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008347496114199935,"score_gpt":0.1993303541766742,"score_spread":0.1909828580624743,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}