{"id":"W4248474704","doi":"10.1093/nar/gnf093","title":"Tripartite molecular beacons","year":2002,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":29,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Molecular beacon; Fluorophore; Nucleic acid; DNA; Biology; Flexibility (engineering); Fluorescence; Nucleic acid thermodynamics; Combinatorial chemistry; Covalent bond; Chromophore; Computational biology; Oligonucleotide; Biochemistry; Base sequence; Chemistry; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005011391,0.0001348953,0.0001406447,0.0001447871,0.0001936715,0.00004835713,0.0002969144,0.0001913657,0.00007233798],"category_scores_gemma":[0.0001856395,0.0001202002,0.0001246566,0.0004030977,0.0003119812,0.000004318053,0.0001967956,0.0002776309,0.0001239946],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002467405,"about_ca_system_score_gemma":0.00001831515,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009258161,"about_ca_topic_score_gemma":0.00000701522,"domain_scores_codex":[0.9982932,0.0001768669,0.0001797879,0.0004435437,0.0004073938,0.0004991752],"domain_scores_gemma":[0.9989675,0.00001745946,0.0000319709,0.0006087038,0.0002345274,0.000139899],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002009891,0.00008738339,0.0002703214,0.00000525905,0.00003825164,0.00002090632,0.00002221337,0.000001717948,0.9660925,0.0002286236,0.02060691,0.01260579],"study_design_scores_gemma":[0.0002303058,0.0002766999,0.0001056768,0.00001035664,0.000009049287,0.0000170432,0.00006316179,0.0002884241,0.7770573,0.000267329,0.2215026,0.0001720971],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9606404,0.001107133,0.001336329,0.00119184,0.00004342477,0.0002354693,0.00001440854,0.0000918605,0.03533911],"genre_scores_gemma":[0.98904,0.0004532904,0.003689408,0.0002471916,0.000153462,0.00001603291,0.00002649934,0.00002943544,0.006344637],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2008957,"threshold_uncertainty_score":0.490162,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04368319130969557,"score_gpt":0.3524321982671834,"score_spread":0.3087490069574879,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}