{"id":"W4249080203","doi":"10.2174/09298665130114","title":"Mutation Studies in the Active Site of &amp;beta;-glycosidase from Pyrococcus furiosus DSM 3638","year":2012,"lang":"en","type":"article","venue":"Protein and Peptide Letters","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"","keywords":"Pyrococcus furiosus; Glycoside hydrolase; Enzyme kinetics; Isothermal titration calorimetry; Thermostability; Active site; Chemistry; Hydrolase; Biochemistry; Thermophile; Enzyme; Mutant","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001526564,0.00009119651,0.00009072555,0.00003095611,0.00004142853,0.00001022066,0.0000478371,0.00004595133,0.00000857584],"category_scores_gemma":[0.00005269917,0.00006979003,0.00002791019,0.00006099282,0.0000784911,0.00001495975,0.00002902816,0.00007130118,0.000005392345],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007340329,"about_ca_system_score_gemma":0.000005152407,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005033215,"about_ca_topic_score_gemma":0.00004335307,"domain_scores_codex":[0.9994087,0.0001012667,0.0001370335,0.0001489494,0.00008091373,0.0001231027],"domain_scores_gemma":[0.9997007,0.00001404196,0.00009002748,0.0001408605,0.00003125149,0.00002317785],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000684534,0.00003320915,0.003583644,0.00002545807,0.00003024968,7.602247e-7,0.002528114,0.000004279601,0.9917846,0.00001613119,0.0004736056,0.001451445],"study_design_scores_gemma":[0.0004744056,0.00005162191,0.04906442,0.00003672845,0.00002071501,0.000007657633,0.0007761494,0.000002263905,0.937387,0.00004473023,0.01198979,0.0001444429],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9961571,0.001004349,0.000236298,0.002183819,0.00007435853,0.0002764545,0.00001916764,0.000004938071,0.00004358169],"genre_scores_gemma":[0.9977635,0.0001064433,0.0003302556,0.001220818,0.0002749394,0.00006232788,0.000166639,0.000007731034,0.00006730597],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05439758,"threshold_uncertainty_score":0.2845955,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02881241954372139,"score_gpt":0.2853514123237706,"score_spread":0.2565389927800492,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}