{"id":"W4250767639","doi":"10.1128/aem.02754-07","title":"Design and Performance of a 16S rRNA-Targeted Oligonucleotide Probe for Detection of Members of the Genus <i>Bdellovibrio</i> by Fluorescence In Situ Hybridization","year":2008,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Vibrio bacteria research studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"Western University","funders":"","keywords":"Oligonucleotide; Biology; Bdellovibrio; In situ; 16S ribosomal RNA; Fluorescence in situ hybridization; In situ hybridization; Ribosomal RNA; Molecular probe; Oligomer restriction; Nucleic acid thermodynamics; Molecular biology; Bacteria; Microbiology; Genetics; DNA; Base sequence; Chemistry; Gene; Messenger RNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009792271,0.001009696,0.001008585,0.0005321098,0.0007752299,0.0006123739,0.0009373057,0.001034031,0.001640846],"category_scores_gemma":[0.001079471,0.001313699,0.0009446839,0.0004659236,0.0007209016,0.0005472435,0.0004584685,0.00119237,0.001307623],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008642417,"about_ca_system_score_gemma":0.00132666,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001523625,"about_ca_topic_score_gemma":0.00221136,"domain_scores_codex":[0.9989262,0.0001623927,0.00008861641,0.0004177554,0.0002416363,0.000163414],"domain_scores_gemma":[0.9993966,0.0001810284,0.00009178816,0.00005318963,0.0001839792,0.00009350253],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002955426,0.00001023817,0.00003460414,0.00002389887,0.00000186263,0.000006816948,0.00001568109,0.0000583394,0.9992703,0.00007072632,0.00001574717,0.000462257],"study_design_scores_gemma":[0.00002727391,0.0002854616,0.0005866091,0.000008046176,0.0000198247,0.00008591645,0.00002558201,0.001315708,0.9958076,0.00009504308,0.001731684,0.0000110915],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6500314,0.001145894,0.3397278,0.0008096404,0.000258967,0.001005059,0.001537858,0.001437436,0.004045872],"genre_scores_gemma":[0.4478921,0.001291849,0.5329456,0.0006498416,0.00009301429,0.002077679,0.005176201,0.0003964155,0.009477274],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001640846,"threshold_uncertainty_score":0.006270528,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004967110732025651,"score_gpt":0.1748910374964395,"score_spread":0.1699239267644139,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}