{"id":"W4250829258","doi":"10.1042/bj3490141","title":"Expression, purification and characterization of recombinant human choline acetyltransferase: phosphorylation of the enzyme regulates catalytic activity","year":2000,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":27,"is_retracted":false,"has_abstract":true,"ca_institutions":"Robarts Clinical Trials; Western University","funders":"","keywords":"Biochemistry; Biology; Molecular biology; Enzyme; Protein kinase C; Phosphatase; Choline acetyltransferase; Phosphorylation; Protein kinase A; Kinase; MAP2K7; Dephosphorylation; Cyclin-dependent kinase 2; Cholinergic","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000294473,0.0004735407,0.0004055531,0.0001811759,0.0001344445,0.0003504364,0.0002322683,0.0003079911,0.0005950789],"category_scores_gemma":[0.000288288,0.000169365,0.0003201133,0.0004762909,0.0001251784,0.0001940732,0.00016286,0.0004891248,0.0009966918],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004222872,"about_ca_system_score_gemma":0.0003235441,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002094107,"about_ca_topic_score_gemma":0.001533893,"domain_scores_codex":[0.9998232,0.00002218766,0.00002679724,0.00004018356,0.00005728112,0.00003037177],"domain_scores_gemma":[0.9998729,0.00001253348,0.00001938347,0.00002306492,0.00004864957,0.00002338021],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005720048,0.00001857382,0.0002279236,0.00006350129,0.000008755288,0.00005660583,0.00001984563,0.00005921817,0.9979607,0.00003997905,0.00005797445,0.001429707],"study_design_scores_gemma":[0.00003688381,0.0002171902,0.00754922,0.00001922321,0.00005155041,0.0007686788,0.00004058252,0.001229678,0.9759617,0.00005462934,0.01405886,0.00001175656],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9662955,0.009351153,0.0178826,0.0003756569,0.0001165921,0.0002018028,0.003592303,0.0001571177,0.00202722],"genre_scores_gemma":[0.9305169,0.007556561,0.02794712,0.0001799867,0.00007769132,0.0001412268,0.02029021,0.0001360311,0.01315445],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002094107,"threshold_uncertainty_score":0.004163802,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007621669905852087,"score_gpt":0.2260579606582732,"score_spread":0.2184362907524211,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}