{"id":"W4251421739","doi":"10.2144/02333st03_11824a","title":"Resuspension of DNA Sequencing Reaction Products in Agarose Increases Sequence Quality on an Automated Sequencer","year":2002,"lang":"en","type":"article","venue":"BioTechniques","topic":"Microbial Community Ecology and Physiology","field":"Environmental Science","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"BC Cancer Agency","funders":"","keywords":"Alkaline lysis; DNA; DNA sequencing; Plasmid; Sequence (biology); Agarose; Lysis; Agarose gel electrophoresis; Sequencing by ligation; Sequence analysis; Chromatography; Biology; Computational biology; Chemistry; Base sequence; Molecular biology; Genetics; Genomic library","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003659887,0.0009006272,0.001336836,0.001396851,0.0006476645,0.001679078,0.001051202,0.0008209379,0.006471659],"category_scores_gemma":[0.005921036,0.0006462966,0.000919516,0.0009826683,0.0005116604,0.001045271,0.0006920627,0.001896378,0.004193668],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003600126,"about_ca_system_score_gemma":0.0006755128,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000633901,"about_ca_topic_score_gemma":0.002471855,"domain_scores_codex":[0.997538,0.0007340439,0.00038452,0.0004375934,0.0007389204,0.0001668724],"domain_scores_gemma":[0.994581,0.002450627,0.000778877,0.0008235545,0.001147266,0.0002186727],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007916048,0.00008207401,0.0004292594,0.0001060655,0.0000234852,0.00006405667,0.00007510032,0.000266741,0.9828823,0.0002336885,0.0002098545,0.01554824],"study_design_scores_gemma":[0.00002601552,0.0002417221,0.00214455,0.00003221102,0.00006088514,0.0002204444,0.00003418241,0.003553957,0.985521,0.0003083243,0.007832161,0.00002462935],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3407443,0.001295723,0.6435851,0.0007532441,0.0002434383,0.0007133092,0.0009314554,0.00599594,0.005737471],"genre_scores_gemma":[0.1272109,0.001835028,0.8568263,0.0002757215,0.00005068545,0.0003625376,0.00397666,0.001331552,0.008130596],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.006471659,"threshold_uncertainty_score":0.0216499,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08783226867126823,"score_gpt":0.3317421459932662,"score_spread":0.243909877321998,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}