{"id":"W4254594680","doi":"10.3389/fpls.2018.01737","title":"Expanding the Plant GSTome Through Directed Evolution: DNA Shuffling for the Generation of New Synthetic Enzymes With Engineered Catalytic and Binding Properties","year":2018,"lang":"en","type":"article","venue":"Frontiers in Plant Science","topic":"Glutathione Transferases and Polymorphisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Institute of Population and Public Health; King Saud University","keywords":"DNA shuffling; Biochemistry; Directed evolution; Enzyme; Biology; Protein engineering; Glutathione S-transferase; Gene; Enzyme kinetics; Glutathione; Chemistry; Molecular biology; Active site","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003952114,0.0005988123,0.0004061164,0.0005358679,0.0001287265,0.0004413093,0.0003938312,0.0004000167,0.0005033145],"category_scores_gemma":[0.0002898874,0.0001967493,0.0003668079,0.0006505675,0.0002518136,0.0002796164,0.000560835,0.000748187,0.0003040899],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000290677,"about_ca_system_score_gemma":0.0002229134,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002204115,"about_ca_topic_score_gemma":0.0004063278,"domain_scores_codex":[0.9997979,0.00004691882,0.00001809377,0.00005004963,0.00005808711,0.00002895869],"domain_scores_gemma":[0.9999086,0.0000235815,0.0000319043,0.00001297279,0.000008595233,0.00001439299],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007878082,0.00005406751,0.0003614871,0.0001036044,0.0000229855,0.0001105357,0.00003481847,0.0005456746,0.98689,0.0006366521,0.00004857713,0.01111284],"study_design_scores_gemma":[0.00004341078,0.0004229961,0.001834675,0.00001895041,0.00008796306,0.0006655333,0.00003967654,0.006550109,0.9784144,0.0004311471,0.01147364,0.0000176239],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8859429,0.002661194,0.1078442,0.0002941338,0.00006662925,0.0002208655,0.0005797505,0.0004197674,0.001970551],"genre_scores_gemma":[0.8935882,0.004127122,0.09684435,0.00012649,0.00002475032,0.0001735389,0.001555115,0.0001627659,0.003397837],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005988123,"threshold_uncertainty_score":0.002108991,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02494712035982937,"score_gpt":0.2226693671297246,"score_spread":0.1977222467698952,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}