{"id":"W4256072727","doi":"10.17504/protocols.io.sdpea5n","title":"Western Blot Protocol (Cell Lysate) and Creating Running Gel v1","year":2018,"lang":"en","type":"preprint","venue":"","topic":"Phagocytosis and Immune Regulation","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia, Okanagan Campus; University of British Columbia","funders":"","keywords":"Western blot; Lysis; Protocol (science); Cell; Blot; Computer science; Cell biology; Molecular biology; Chemistry; Biology; Biochemistry; Medicine; Gene; Pathology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003780787,0.006555707,0.005687708,0.004712918,0.003481399,0.003154687,0.005323651,0.002416026,0.09234241],"category_scores_gemma":[0.003660532,0.003150165,0.002625162,0.005056005,0.001745642,0.002334004,0.00198981,0.01168176,0.0733486],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001441305,"about_ca_system_score_gemma":0.002867888,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001986263,"about_ca_topic_score_gemma":0.004820186,"domain_scores_codex":[0.9942002,0.0008092265,0.0008235942,0.001743242,0.001623612,0.0008001783],"domain_scores_gemma":[0.9972031,0.0006024172,0.0001628537,0.0009836212,0.0008375364,0.0002105349],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.001637316,0.0008316794,0.0006190811,0.004893898,0.0003650059,0.0005254772,0.000676959,0.0004922372,0.8648681,0.005763461,0.09361419,0.02571262],"study_design_scores_gemma":[0.0003626216,0.0004486999,0.003888918,0.0004789274,0.000466329,0.001198049,0.000149102,0.001872428,0.4643854,0.003412535,0.5230511,0.0002860009],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.03338172,0.008319315,0.6911263,0.002719913,0.005793891,0.01552737,0.1402261,0.04528861,0.05761692],"genre_scores_gemma":[0.02657535,0.008799548,0.538703,0.001834324,0.001455468,0.03564039,0.2930735,0.013875,0.08004341],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.09234241,"threshold_uncertainty_score":0.3089162,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02428717976876658,"score_gpt":0.2901416046518003,"score_spread":0.2658544248830337,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}