{"id":"W4281550936","doi":"10.1007/s00438-022-01906-1","title":"MS-HRM protocol: a simple and low-cost approach for technical validation of next-generation methylation sequencing data","year":2022,"lang":"en","type":"article","venue":"Molecular Genetics and Genomics","topic":"Epigenetics and DNA Methylation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"McGill University; McGill University Health Centre","funders":"Mashhad University of Medical Sciences; Iran National Science Foundation","keywords":"DNA methylation; Biology; Epigenetics; Methylation; High Resolution Melt; DNA sequencing; Computational biology; Differentially methylated regions; Genetics; DNA; Gene; Polymerase chain reaction; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.004787372,0.003121751,0.002098192,0.004436127,0.002530355,0.001632308,0.003077663,0.002828191,0.02259138],"category_scores_gemma":[0.0090629,0.0023063,0.001377817,0.002000604,0.001327635,0.001702518,0.001729418,0.005639047,0.02915078],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009535672,"about_ca_system_score_gemma":0.00395049,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001976401,"about_ca_topic_score_gemma":0.005018399,"domain_scores_codex":[0.9957364,0.0009972049,0.0005390815,0.001190795,0.001123511,0.0004129546],"domain_scores_gemma":[0.9956911,0.001104133,0.0003597674,0.00118549,0.001379654,0.0002799428],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005338538,0.0001569506,0.001017106,0.0005495949,0.0001567396,0.0004399227,0.0002560362,0.0005177213,0.9459882,0.002119792,0.01701352,0.03125058],"study_design_scores_gemma":[0.0001666605,0.0003499824,0.003482018,0.0001457158,0.0001697592,0.001346081,0.0001039913,0.008747879,0.8630021,0.002165256,0.1200785,0.0002420789],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.02893429,0.001340911,0.9057258,0.0006372731,0.0007333919,0.004907915,0.01945145,0.03081582,0.007453125],"genre_scores_gemma":[0.03848836,0.001111766,0.876074,0.001843427,0.0002047137,0.01323501,0.04484947,0.007483972,0.01670927],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.02259138,"threshold_uncertainty_score":0.07557571,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07249613784019353,"score_gpt":0.3127674882099326,"score_spread":0.240271350369739,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}