{"id":"W4281729355","doi":"10.1101/2022.06.10.495194","title":"Identification, expression, and purification of DNA cytosine 5-methyltransferases with short recognition sequences","year":2022,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Epigenetics and DNA Methylation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Infection and Immunity","funders":"Japan Society for the Promotion of Science; Japan Agency for Medical Research and Development","keywords":"DNA methylation; Epigenetics; Methyltransferase; DNA methyltransferase; DNA; Recognition sequence; Biology; Genetics; Epigenomics; Methylation; Computational biology; DNA sequencing; CpG site; Cytosine; Gene; Restriction enzyme; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004607912,0.0005189478,0.0004322109,0.0004355799,0.0001989392,0.0005214315,0.0003381241,0.0004057844,0.000618202],"category_scores_gemma":[0.0005269104,0.0002054452,0.0004323159,0.0005215834,0.0002486358,0.0002197712,0.0003226271,0.0006514215,0.0007718976],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003807216,"about_ca_system_score_gemma":0.0004158157,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001132259,"about_ca_topic_score_gemma":0.001455515,"domain_scores_codex":[0.9996656,0.00005737016,0.00004165628,0.00008622603,0.00008344556,0.00006560644],"domain_scores_gemma":[0.9997566,0.00005246116,0.00004744848,0.00004124349,0.0000486198,0.00005356253],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005705257,0.00002403477,0.0004360461,0.0000336597,0.000006428963,0.00002503856,0.00001017121,0.0000733223,0.9974336,0.0000919708,0.00004117793,0.001767515],"study_design_scores_gemma":[0.00001399818,0.0001034851,0.002535719,0.000007415195,0.00003052306,0.0001974821,0.00002250144,0.001210697,0.9921726,0.00008572373,0.003615243,0.000004670568],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9469911,0.005412861,0.04359591,0.0003428989,0.00007137759,0.0001905611,0.001647865,0.0001297931,0.001617757],"genre_scores_gemma":[0.9134971,0.002365233,0.06573529,0.0001151726,0.00005149994,0.0001620342,0.01206833,0.00009003978,0.005915203],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001132259,"threshold_uncertainty_score":0.002762377,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01604811174951839,"score_gpt":0.2356345262904316,"score_spread":0.2195864145409132,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}