{"id":"W4281734206","doi":"10.1101/2022.06.08.495323","title":"Improved protein glycosylation enabled heterologous biosynthesis of monoterpenoid indole alkaloids and their unnatural derivatives in yeast","year":2022,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Alkaloids: synthesis and pharmacology","field":"Pharmacology, Toxicology and Pharmaceutics","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; University of New Brunswick","funders":"","keywords":"Catharanthus roseus; Biosynthesis; Biochemistry; Biology; Indole alkaloid; Vindoline; Indole test; Chemistry; Vinblastine; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow","research_integrity"],"consensus_categories":[],"category_scores_codex":[0.001792439,0.0008665853,0.001233284,0.0006742437,0.0002997892,0.00006403014,0.0008077284,0.001266606,0.0007787629],"category_scores_gemma":[0.0003246408,0.0008597291,0.0002283125,0.0005770391,0.0004771386,0.0002047309,0.001062123,0.002580581,0.000009194164],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003550861,"about_ca_system_score_gemma":0.000449976,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001838569,"about_ca_topic_score_gemma":0.00001735931,"domain_scores_codex":[0.9947613,0.001788624,0.001123216,0.001224945,0.0002255124,0.0008763947],"domain_scores_gemma":[0.9973059,0.0004895871,0.001008326,0.0006645284,0.000236584,0.0002951233],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0009814487,0.0004968228,0.01180965,0.0004513855,0.0004580841,0.00003560048,0.0002209452,0.0002250202,0.9850547,0.0001416729,0.0000353501,0.00008927162],"study_design_scores_gemma":[0.001733616,0.0002394065,0.05476761,0.0001144397,0.0001621491,1.166529e-7,0.00003808717,0.004206946,0.9346694,0.00002046243,0.003208193,0.0008395183],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9925829,0.002833959,0.0001489288,0.0003396035,0.001061436,0.002109313,0.0007259608,0.0001796474,0.00001826332],"genre_scores_gemma":[0.9969971,0.0005285137,0.0007033469,0.0004269342,0.0002529019,0.0009393108,0.000002967061,0.000138806,0.00001017932],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05038529,"threshold_uncertainty_score":0.9997205,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03469852854192537,"score_gpt":0.3022498982943585,"score_spread":0.2675513697524332,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}