{"id":"W4282559081","doi":"10.1101/2022.06.12.495817","title":"Divergent Directed Evolution of a TetR-type Repressor Towards Aromatic Molecules","year":2022,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University; PROTEO","funders":"Fonds de recherche du Québec – Nature et technologies; Natural Sciences and Engineering Research Council of Canada; Concordia University","keywords":"TetR; Computational biology; Small molecule; Repressor; Biology; Allosteric regulation; Synthetic biology; Biochemistry; Transcription factor; Chemistry; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005265963,0.0003613886,0.000328699,0.00032345,0.0001514444,0.0006244266,0.0005618433,0.0003594387,0.0009720158],"category_scores_gemma":[0.0004285462,0.0001877022,0.0003134195,0.0003714053,0.0003447666,0.000163878,0.0005923722,0.001016055,0.0007244415],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006292085,"about_ca_system_score_gemma":0.0004308509,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001556166,"about_ca_topic_score_gemma":0.001877081,"domain_scores_codex":[0.9996912,0.00006027284,0.00002737892,0.00007811945,0.00009360773,0.00004944338],"domain_scores_gemma":[0.9997829,0.00006088361,0.00004117737,0.00003816043,0.00003800679,0.00003894994],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000710992,0.00004886945,0.0003198517,0.00002648534,0.000009154807,0.00008384872,0.00003046685,0.0005527493,0.9934235,0.001031999,0.0001022817,0.004299739],"study_design_scores_gemma":[0.00002295086,0.0001408993,0.001244307,0.00001054061,0.00002055061,0.0002915266,0.00006035985,0.008517958,0.9830336,0.000266531,0.006371269,0.00001953593],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9496508,0.0004277532,0.04412609,0.0003386521,0.00011161,0.00008833838,0.0007281227,0.0006152143,0.003913343],"genre_scores_gemma":[0.9623763,0.0004183087,0.02901691,0.0001196395,0.00001924679,0.00004582751,0.000933555,0.0003669105,0.006703185],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001556166,"threshold_uncertainty_score":0.004565179,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00821605259357834,"score_gpt":0.2079656801963066,"score_spread":0.1997496276027283,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}