{"id":"W4282823769","doi":"10.3389/fbioe.2022.887716","title":"Production of Lentiviral Vectors Using a HEK-293 Producer Cell Line and Advanced Perfusion Processing","year":2022,"lang":"en","type":"article","venue":"Frontiers in Bioengineering and Biotechnology","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"National Research Council Canada; Fonds de recherche du Québec; Canada Research Chairs","keywords":"HEK 293 cells; Viral vector; Perfusion; Cell culture; Line (geometry); Chemistry; Cell biology; Biology; Medicine; Internal medicine; Recombinant DNA; Genetics; Biochemistry; Mathematics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007183443,0.000454131,0.000644366,0.0003464645,0.0003383403,0.0008803445,0.000625251,0.0006572132,0.001422715],"category_scores_gemma":[0.000499882,0.0003006935,0.0004466848,0.0004347262,0.0003430901,0.0004829471,0.0003843862,0.001291399,0.002192143],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003809161,"about_ca_system_score_gemma":0.0004164528,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006303245,"about_ca_topic_score_gemma":0.0005958251,"domain_scores_codex":[0.9995617,0.00006014624,0.00006660611,0.00008942546,0.000180809,0.00004135459],"domain_scores_gemma":[0.9998343,0.00003932981,0.0000337866,0.00003185183,0.00004283529,0.00001794177],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004042815,0.00003450019,0.0001582105,0.00004919554,0.000004643391,0.00009653972,0.00003375992,0.0001546044,0.9956169,0.0002771162,0.0001258825,0.003408183],"study_design_scores_gemma":[0.00001960704,0.0001698578,0.0007133235,0.00001219946,0.00001845417,0.000366427,0.00001898865,0.002358277,0.9875191,0.0001415041,0.008647006,0.00001513035],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4725929,0.002893207,0.5039053,0.0004364059,0.0003944467,0.002212339,0.004653763,0.002221036,0.01069058],"genre_scores_gemma":[0.5884622,0.004696887,0.3794279,0.0002942639,0.0001025855,0.001518621,0.009377582,0.0005477357,0.01557225],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001422715,"threshold_uncertainty_score":0.004759431,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00781223162690049,"score_gpt":0.2356821960294959,"score_spread":0.2278699644025954,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}