{"id":"W4283384326","doi":"10.1016/j.ymthe.2022.06.012","title":"Lipid nanoparticle-mediated silencing of osteogenic suppressor GNAS leads to osteogenic differentiation of mesenchymal stem cells in vivo","year":2022,"lang":"en","type":"article","venue":"Molecular Therapy","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":false,"ca_institutions":"Simon Fraser University; NanoMedicines Innovation Network; University of British Columbia","funders":"Canadian Institutes of Health Research; Crohn's and Colitis Canada; Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung","keywords":"Mesenchymal stem cell; Gene silencing; Osteoblast; GNAS complex locus; Small interfering RNA; Cell biology; Transfection; Cancer research; RNA interference; Osteoporosis; Biology; In vitro; Cell culture; RNA; Endocrinology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002136863,0.0001775892,0.000261906,0.000143088,0.0000441027,0.000008221882,0.0003470107,0.00008315143,0.0001940957],"category_scores_gemma":[0.000011777,0.0001853011,0.0001679629,0.0002783618,0.00003508382,0.000005606623,0.0001827165,0.00009730639,0.000005639019],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002872324,"about_ca_system_score_gemma":0.00007124306,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005103149,"about_ca_topic_score_gemma":0.00002239008,"domain_scores_codex":[0.9984032,0.0002351561,0.0004335642,0.0003607836,0.0002878906,0.0002794208],"domain_scores_gemma":[0.9992409,0.00001791848,0.0001602837,0.000425868,0.0000896015,0.00006538005],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000456973,0.0001624024,0.002508965,0.00001516076,0.00006571537,0.000006427341,0.0004402442,0.002332109,0.9929876,0.000006309716,0.00009056361,0.0009275396],"study_design_scores_gemma":[0.00101154,0.001394933,0.0008191118,0.00001346944,0.00001268072,0.000003834856,0.0002653424,0.00015911,0.9957667,0.000006823414,0.0003624319,0.0001840392],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9980593,0.0009731714,0.0002111534,0.00006056369,0.0001779791,0.0003797696,0.00008902269,0.000007493752,0.00004153564],"genre_scores_gemma":[0.9992914,0.0001086155,0.00004720789,0.0002234803,0.00002658868,0.00007968359,0.00004230212,0.00002906517,0.0001516935],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002779094,"threshold_uncertainty_score":0.7556362,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01008790361406991,"score_gpt":0.2259775270230094,"score_spread":0.2158896234089394,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}