{"id":"W4283384326","doi":"10.1016/j.ymthe.2022.06.012","title":"Lipid nanoparticle-mediated silencing of osteogenic suppressor GNAS leads to osteogenic differentiation of mesenchymal stem cells in vivo","year":2022,"lang":"en","type":"article","venue":"Molecular Therapy","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":false,"ca_institutions":"Simon Fraser University; NanoMedicines Innovation Network; University of British Columbia","funders":"Canadian Institutes of Health Research; Crohn's and Colitis Canada; Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung","keywords":"Mesenchymal stem cell; Gene silencing; Osteoblast; GNAS complex locus; Small interfering RNA; Cell biology; Transfection; Cancer research; RNA interference; Osteoporosis; Biology; In vitro; Cell culture; RNA; Endocrinology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001139946,0.0002550283,0.0002121065,0.0001653376,0.0001246114,0.0002145338,0.0001504335,0.0002824544,0.0008668342],"category_scores_gemma":[0.00007330949,0.0001320644,0.0002418641,0.0001239334,0.0002148529,0.0001275542,0.0001739507,0.000363231,0.000264409],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000240173,"about_ca_system_score_gemma":0.0002340332,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009194788,"about_ca_topic_score_gemma":0.001429203,"domain_scores_codex":[0.9998912,0.00000961875,0.00000803945,0.00002307018,0.00004459617,0.00002356892],"domain_scores_gemma":[0.9999422,0.00001214922,0.00001898584,0.00000621903,0.000007435159,0.00001313494],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003006104,0.000008507098,0.00002187446,0.00000664025,0.000001480241,0.00001777884,0.00000569314,0.00004027279,0.9994915,0.00005737663,0.00001040829,0.0003084966],"study_design_scores_gemma":[0.000005707395,0.00004931838,0.0002803394,7.218625e-7,0.00000548109,0.00003116392,0.000006836214,0.0004199241,0.9987323,0.00002018796,0.0004465952,0.000001417046],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9921384,0.0004399559,0.004577556,0.00008833354,0.00003975096,0.00002772163,0.0001469983,0.00009715522,0.002444133],"genre_scores_gemma":[0.9932672,0.0003093706,0.001667133,0.00003687396,0.00000360121,0.00002170265,0.0001437379,0.00002716612,0.0045232],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009194788,"threshold_uncertainty_score":0.002899826,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01008790361406991,"score_gpt":0.2259775270230094,"score_spread":0.2158896234089394,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}