{"id":"W4283745052","doi":"10.3791/63977","title":"Nonspecific Proteolysis-Based Glycomics Strategy for Bacterial Glycoproteins","year":2022,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada","funders":"","keywords":"Glycan; Campylobacter jejuni; Glycomics; Glycoprotein; Glycosylation; Biochemistry; Exoglycosidase; PNGase F; N-linked glycosylation; Endoglycosidase; Chemistry; Biology; Bacteria","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003281494,0.0007271078,0.0004849269,0.0002183728,0.0003289475,0.000250419,0.0003868732,0.0004550967,0.0004710131],"category_scores_gemma":[0.0001488185,0.0002296418,0.000380494,0.0002990413,0.0002473609,0.0002422587,0.0003903995,0.0008396471,0.0004193098],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003523038,"about_ca_system_score_gemma":0.0002965113,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005802881,"about_ca_topic_score_gemma":0.001275319,"domain_scores_codex":[0.9997001,0.00006158127,0.00001762799,0.0000893807,0.00008538939,0.00004594908],"domain_scores_gemma":[0.9999293,0.00001089719,0.00001625048,0.00001427407,0.00001859261,0.00001076725],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002117728,0.000009082664,0.00004610328,0.00005516488,0.000004572978,0.00003489666,0.00001197531,0.00002640461,0.9985586,0.0001077806,0.00004948439,0.001074827],"study_design_scores_gemma":[0.000004334952,0.00009395918,0.0008636022,0.000008356692,0.0000134459,0.000360881,0.00001462494,0.001006437,0.9929864,0.00008520688,0.004555528,0.000007088864],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6955839,0.009542858,0.2852553,0.0006298605,0.000223119,0.0006947601,0.001054194,0.00041922,0.00659686],"genre_scores_gemma":[0.737313,0.0118751,0.2336845,0.0008022258,0.00006896674,0.0007428838,0.002490795,0.000155142,0.01286746],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.0007271078,"threshold_uncertainty_score":0.002556205,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04786435836066856,"score_gpt":0.427651633154444,"score_spread":0.3797872747937755,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}