{"id":"W4285047717","doi":"10.21769/bioprotoc.4419","title":"Purification of the Bacterial Amyloid “Curli” from Salmonella enterica Serovar Typhimurium and Detection of Curli from Infected Host Tissues","year":2022,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Salmonella and Campylobacter epidemiology","field":"Agricultural and Biological Sciences","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan","funders":"National Institute of Allergy and Infectious Diseases","keywords":"Salmonella enterica; Biofilm; Biology; Microbiology; Escherichia coli; Innate immune system; Context (archaeology); Bacterial cell structure; Salmonella; Amyloid (mycology); Bacteria; Mutant; Immune system; Biochemistry; Gene; Immunology; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004445016,0.0008009364,0.0002762543,0.0004166033,0.0004157701,0.0002774574,0.000336859,0.0004337673,0.002036003],"category_scores_gemma":[0.0003914991,0.0003320552,0.0004808082,0.0003448405,0.0002872739,0.0002864278,0.0003738758,0.000969933,0.00176435],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002253394,"about_ca_system_score_gemma":0.0003383284,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00100485,"about_ca_topic_score_gemma":0.002100713,"domain_scores_codex":[0.9996068,0.00007724737,0.00004405164,0.00008576318,0.000106505,0.00007960937],"domain_scores_gemma":[0.9995465,0.00007395461,0.00007932447,0.00008921658,0.000131394,0.00007952406],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003357646,0.000008650364,0.00011498,0.00006667939,0.000003029988,0.00003745883,0.00002295427,0.00001702722,0.9989442,0.00006315576,0.0001198351,0.0005684283],"study_design_scores_gemma":[0.000009922465,0.0003159038,0.005185999,0.0000269296,0.00002200636,0.0006447519,0.00006187228,0.0004812001,0.983332,0.0001127644,0.009791798,0.00001482145],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7375858,0.003566891,0.2396174,0.001120299,0.0005126714,0.001518086,0.005039745,0.001719865,0.009319293],"genre_scores_gemma":[0.5484612,0.0041181,0.3811808,0.001088111,0.00009059539,0.002092915,0.02833463,0.0008333738,0.03380025],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002036003,"threshold_uncertainty_score":0.006811142,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02104772754121299,"score_gpt":0.2483886456660838,"score_spread":0.2273409181248708,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}