{"id":"W4285047717","doi":"10.21769/bioprotoc.4419","title":"Purification of the Bacterial Amyloid “Curli” from Salmonella enterica Serovar Typhimurium and Detection of Curli from Infected Host Tissues","year":2022,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Salmonella and Campylobacter epidemiology","field":"Agricultural and Biological Sciences","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan","funders":"National Institute of Allergy and Infectious Diseases","keywords":"Salmonella enterica; Biofilm; Biology; Microbiology; Escherichia coli; Innate immune system; Context (archaeology); Bacterial cell structure; Salmonella; Amyloid (mycology); Bacteria; Mutant; Immune system; Biochemistry; Gene; Immunology; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.0002079678,0.0001594423,0.0002886026,0.00001561479,0.0001905807,0.0000192345,0.0003205903,0.0001141159,0.001088806],"category_scores_gemma":[0.00007232773,0.00006744493,0.00009871324,0.0002899138,0.0001337766,0.00006111154,0.0002524959,0.0001374847,0.000006763474],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002866681,"about_ca_system_score_gemma":0.00001122705,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003748549,"about_ca_topic_score_gemma":0.0002412047,"domain_scores_codex":[0.9983212,0.0005194641,0.0004440227,0.0003525966,0.0001866144,0.0001760534],"domain_scores_gemma":[0.9990317,0.000232287,0.0004627436,0.0001589973,0.00006319781,0.00005103846],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"observational","study_design_scores_codex":[0.0003576055,0.0001096206,0.04987037,0.000008486561,0.00001888631,2.974507e-7,0.00008944772,6.758597e-7,0.9412615,0.000008688326,0.0001306142,0.008143793],"study_design_scores_gemma":[0.0004207498,0.000422854,0.7173136,0.00001250412,0.00001607359,0.000001823556,0.0001203954,0.00002674344,0.2251006,0.0002766734,0.05616632,0.0001216466],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9376327,0.00001193487,0.000004076105,0.0002286383,0.0002086693,0.06146334,0.0003459998,0.00003506757,0.00006955218],"genre_scores_gemma":[0.8928759,0.000003019546,0.00004363618,0.00005732535,0.0002589733,0.1066397,0.00009216047,0.000002194916,0.00002714009],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.7161609,"threshold_uncertainty_score":0.9998243,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02104772754121299,"score_gpt":0.2483886456660838,"score_spread":0.2273409181248708,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}