{"id":"W4285079241","doi":"10.1007/978-1-0716-2209-4_7","title":"Imaging Insulin Granule Dynamics in Human Pancreatic β-Cells Using Total Internal Reflection Fluorescence (TIRF) Microscopy","year":2022,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Pancreatic function and diabetes","field":"Medicine","cited_by":3,"is_retracted":false,"has_abstract":false,"ca_institutions":"Toronto General Hospital; University of Toronto","funders":"Canadian Institutes of Health Research","keywords":"Total internal reflection fluorescence microscope; Granule (geology); Microscope; Fluorescence microscope; Microscopy; Materials science; Biophysics; Optics; Chemistry; Fluorescence; Biology; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001921298,0.0001707529,0.0002046955,0.000152421,0.000185871,0.0003755932,0.0002525431,0.0004022714,0.000671764],"category_scores_gemma":[0.0001723062,0.0001639949,0.0002496254,0.0002028829,0.0001556457,0.0003263157,0.0002144013,0.0005443642,0.0003293837],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002354342,"about_ca_system_score_gemma":0.0001495073,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001204676,"about_ca_topic_score_gemma":0.0008693698,"domain_scores_codex":[0.9999225,0.00001049324,0.000004309486,0.00002531886,0.00001973265,0.00001764022],"domain_scores_gemma":[0.9999233,0.00002497756,0.00001258286,0.00001416082,0.00001520698,0.00000957353],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007224298,0.000007949939,0.0002832522,0.00002427453,0.00000371927,0.00005415846,0.00004338696,0.0001700683,0.9970114,0.0000851954,0.00009037861,0.002153876],"study_design_scores_gemma":[0.00005031106,0.0001619123,0.01245693,0.00001282359,0.00002493239,0.0006543748,0.0002049054,0.0146888,0.9683461,0.000274587,0.003100538,0.00002369004],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9285459,0.001514032,0.06555803,0.0003107714,0.00003765715,0.00004030994,0.000562304,0.000441975,0.002988952],"genre_scores_gemma":[0.9383717,0.001895276,0.05655954,0.000149761,0.00001989994,0.00007495976,0.000447157,0.0001315063,0.002350338],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001204676,"threshold_uncertainty_score":0.002395332,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02191737330927546,"score_gpt":0.3968643143727278,"score_spread":0.3749469410634524,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}