{"id":"W4285128366","doi":"10.1007/978-1-0716-2124-0_5","title":"Protein Purification and Digestion Methods for Bacterial Proteomic Analyses","year":2022,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Advanced Proteomics Techniques and Applications","field":"Chemistry","cited_by":3,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Regina","funders":"","keywords":"Proteome; Computational biology; Extraction (chemistry); Chromatography; Chemistry; Biology; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001537133,0.001395972,0.001144753,0.001494924,0.001014225,0.0007151987,0.001226428,0.0008031394,0.002342565],"category_scores_gemma":[0.001702193,0.0008635675,0.0008788175,0.002404861,0.0004497622,0.0006707071,0.0006871111,0.00290798,0.006190927],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005229015,"about_ca_system_score_gemma":0.0009091261,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001177208,"about_ca_topic_score_gemma":0.002463585,"domain_scores_codex":[0.9981669,0.0002809923,0.0001882762,0.0002780107,0.0008843141,0.0002014184],"domain_scores_gemma":[0.9992887,0.0001389589,0.00007164076,0.0001922976,0.0002586988,0.00004973696],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000139527,0.000109952,0.0002539484,0.0007545228,0.00004045554,0.00008197644,0.00007584604,0.0001127757,0.9593713,0.0006973372,0.0042174,0.03414499],"study_design_scores_gemma":[0.00002995758,0.0001507305,0.004158302,0.0001016583,0.00005987337,0.000756233,0.00003630451,0.00111597,0.8667507,0.0008709166,0.125927,0.00004248737],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.05286644,0.02695491,0.8935168,0.001570154,0.001093582,0.001800673,0.00995664,0.003534289,0.008706552],"genre_scores_gemma":[0.06467339,0.03689834,0.8321027,0.001440153,0.0003306326,0.003167523,0.03934493,0.001206167,0.02083614],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002342565,"threshold_uncertainty_score":0.008129239,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04815471487363363,"score_gpt":0.4699312883174653,"score_spread":0.4217765734438316,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}