{"id":"W4285169583","doi":"10.1007/978-1-0716-1979-7_22","title":"In Situ Quantification and Isolation of Müller Glial Cells by Fluorescence-Activated Cell Sorting from the Regenerating Larval Zebrafish Retina","year":2022,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Retinal Development and Disorders","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"","keywords":"Cell sorting; Muller glia; Retina; Zebrafish; Biology; Cell biology; Population; Cell; Molecular biology; Transcriptome; In situ hybridization; Flow cytometry; Genetics; Neuroscience; Gene expression; Stem cell; Progenitor cell; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004125621,0.0006378411,0.00046371,0.001300983,0.00100655,0.0008588069,0.0006175092,0.0006120559,0.002633384],"category_scores_gemma":[0.0002938765,0.0004696026,0.0004408522,0.000692614,0.000488642,0.0004837211,0.0004297339,0.001481097,0.001072117],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009415658,"about_ca_system_score_gemma":0.0008809059,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.006208464,"about_ca_topic_score_gemma":0.01492178,"domain_scores_codex":[0.9996089,0.0000228577,0.00002922031,0.0001146413,0.0001260799,0.00009837565],"domain_scores_gemma":[0.9997434,0.00005528904,0.00006230624,0.0000351947,0.00006525442,0.0000384218],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002268874,0.000007244284,0.0001015513,0.00003087435,0.000002730432,0.00001411542,0.00003303286,0.00003919354,0.998063,0.000255041,0.00006630366,0.001364182],"study_design_scores_gemma":[0.00001589579,0.00004012826,0.004965846,0.00001469955,0.00001703517,0.0000911602,0.00004645176,0.001353771,0.9892111,0.0001267076,0.004109292,0.00000795502],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7197866,0.004153697,0.2550152,0.0007147769,0.000245536,0.0005941139,0.005660656,0.002078453,0.01175086],"genre_scores_gemma":[0.6507145,0.004797026,0.3118667,0.0004939431,0.0001441634,0.001449418,0.004502909,0.00104213,0.02498922],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006208464,"threshold_uncertainty_score":0.0123446,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01343577671908839,"score_gpt":0.30895889742219,"score_spread":0.2955231207031015,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}