{"id":"W4285200886","doi":"10.1007/978-1-0716-2376-3_21","title":"Reverse Transcription-Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) for Gene Expression Analyses","year":2022,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":false,"ca_institutions":"Memorial University of Newfoundland","funders":"","keywords":"Real-time polymerase chain reaction; Reverse transcription polymerase chain reaction; Reverse transcriptase; Gene expression; Biology; Gene; Molecular biology; Polymerase chain reaction; Computational biology; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.004281094,0.007344529,0.006992556,0.004710114,0.003100273,0.002904949,0.004564255,0.00364001,0.02936669],"category_scores_gemma":[0.00239165,0.003726531,0.005033104,0.006993797,0.003125664,0.002510797,0.002098849,0.01102477,0.02615973],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.002127432,"about_ca_system_score_gemma":0.005286098,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002210306,"about_ca_topic_score_gemma":0.006788784,"domain_scores_codex":[0.98717,0.001753755,0.001243355,0.005169547,0.003087855,0.001575589],"domain_scores_gemma":[0.9978985,0.0005928514,0.0001913687,0.0005172027,0.0006789539,0.0001212049],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004246581,0.0004997731,0.0003967859,0.001266554,0.0001070169,0.0001280419,0.0001580164,0.00118128,0.9738555,0.002830853,0.006116222,0.01303533],"study_design_scores_gemma":[0.0002851567,0.0006977753,0.001431069,0.0002264474,0.0002427062,0.0002329655,0.0000844953,0.01053794,0.9109725,0.002585076,0.0724648,0.0002391349],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.02042291,0.002085186,0.9090713,0.0005673708,0.002396161,0.005527834,0.03313743,0.0142028,0.012589],"genre_scores_gemma":[0.02371944,0.002105315,0.8860356,0.001197896,0.0003718042,0.01747514,0.03791949,0.004932593,0.02624281],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.02936669,"threshold_uncertainty_score":0.09824139,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04819367751045631,"score_gpt":0.4450076246101756,"score_spread":0.3968139470997193,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}