{"id":"W4285392129","doi":"10.1186/s12896-022-00750-w","title":"Oleic acid based experimental evolution of Bacillus megaterium yielding an enhanced P450 BM3 variant","year":2022,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"Pharmacogenetics and Drug Metabolism","field":"Pharmacology, Toxicology and Pharmaceutics","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval","funders":"","keywords":"Bacillus megaterium; Monooxygenase; Biology; Cofactor; Directed evolution; Biochemistry; Cytochrome P450; Enzyme; Stereochemistry; Mutant; Chemistry; Bacteria; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow","insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.0007495908,0.0002905174,0.0004156795,0.0004334017,0.0004427931,0.000009195197,0.000716692,0.000548219,0.003314144],"category_scores_gemma":[0.00005012126,0.0003204088,0.0001399052,0.0005249963,0.0003531362,0.00008216988,0.000451376,0.0009484326,0.00003196045],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000219716,"about_ca_system_score_gemma":0.0002394604,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004226901,"about_ca_topic_score_gemma":0.00001100902,"domain_scores_codex":[0.9973958,0.000682271,0.0005161834,0.000571676,0.0002358104,0.0005982668],"domain_scores_gemma":[0.9989393,0.00008728298,0.0002808585,0.0004698193,0.00005862883,0.000164085],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005253611,0.0007443717,0.000192528,0.00002654059,0.00006103783,0.00002465956,0.0002396421,0.0030281,0.9857563,0.005573505,0.0001433342,0.003684637],"study_design_scores_gemma":[0.002324997,0.0005110623,0.0002313975,0.000003364677,0.00009452111,0.00005088766,0.0006825142,0.01783238,0.947364,0.0004018508,0.03018071,0.0003222991],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9685664,0.003451203,0.02337296,0.0004900305,0.0023155,0.0005137182,0.0002116477,0.0002836358,0.0007948636],"genre_scores_gemma":[0.994652,0.00005462016,0.004143147,0.0006172447,0.0001310327,0.0002288551,0.00004566478,0.00003868853,0.00008878313],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03839227,"threshold_uncertainty_score":0.9999248,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07481905090783386,"score_gpt":0.3818607414346483,"score_spread":0.3070416905268145,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}