{"id":"W4285392129","doi":"10.1186/s12896-022-00750-w","title":"Oleic acid based experimental evolution of Bacillus megaterium yielding an enhanced P450 BM3 variant","year":2022,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"Pharmacogenetics and Drug Metabolism","field":"Pharmacology, Toxicology and Pharmaceutics","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval","funders":"","keywords":"Bacillus megaterium; Monooxygenase; Biology; Cofactor; Directed evolution; Biochemistry; Cytochrome P450; Enzyme; Stereochemistry; Mutant; Chemistry; Bacteria; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003016148,0.0004664173,0.0003343054,0.0002108684,0.0001624206,0.0004548998,0.0003375642,0.0003582954,0.0006997305],"category_scores_gemma":[0.0004535738,0.0001429486,0.0003308371,0.0004478709,0.0002254045,0.0001707518,0.0005468858,0.0007203876,0.000281474],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003197476,"about_ca_system_score_gemma":0.0002195285,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007024753,"about_ca_topic_score_gemma":0.0007619776,"domain_scores_codex":[0.9997204,0.00005448857,0.00004283502,0.00006148076,0.00008347615,0.00003746924],"domain_scores_gemma":[0.9998137,0.00003950296,0.00005908308,0.00003786246,0.0000260716,0.00002367166],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001244566,0.00007665082,0.0004527527,0.00006203994,0.00001633203,0.0001042253,0.00002981661,0.000396023,0.9944503,0.0001212427,0.00005277663,0.00411328],"study_design_scores_gemma":[0.00003231011,0.000453853,0.002845502,0.00001523032,0.00004779135,0.0003970592,0.00004044843,0.002675141,0.9895301,0.00005689963,0.003893002,0.00001259714],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9890389,0.0004940028,0.008026704,0.0001839799,0.00003841982,0.00007452468,0.0004427515,0.0001455727,0.001555175],"genre_scores_gemma":[0.980807,0.0005860009,0.01492631,0.00007440658,0.00000873481,0.00007594542,0.001476692,0.0001063904,0.001938437],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007024753,"threshold_uncertainty_score":0.002340853,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07481905090783386,"score_gpt":0.3818607414346483,"score_spread":0.3070416905268145,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}