{"id":"W4285744263","doi":"10.1039/d2cc03079a","title":"Selectivity and efficiency in the ligation of the pyrene:abasic base pair by T4 and PBCV-1 DNA ligases","year":2022,"lang":"en","type":"article","venue":"Chemical Communications","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"AP site; DNA ligase; Pyrene; Ligation; DNA; DNA Ligases; Chemistry; Selectivity; Base excision repair; Base pair; Nucleotide; Biochemistry; DNA repair; Molecular biology; Biology; Gene; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002086549,0.0003228918,0.0005708865,0.0003924381,0.0002123758,0.0009891881,0.0005723434,0.0006957504,0.0005832905],"category_scores_gemma":[0.002108002,0.0004542824,0.0003176317,0.0003020735,0.0004636273,0.0004472287,0.0003575086,0.0006274834,0.0005136004],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000503853,"about_ca_system_score_gemma":0.0002420398,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005285023,"about_ca_topic_score_gemma":0.0006647444,"domain_scores_codex":[0.9979079,0.0005539759,0.0001730901,0.000508083,0.0006042175,0.0002527702],"domain_scores_gemma":[0.9987263,0.0005726498,0.0001910376,0.0001217656,0.0002571079,0.0001310694],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000104817,0.00001078932,0.000222072,0.00002515696,0.000004379264,0.00001748728,0.00002568731,0.000080121,0.998261,0.00006923903,0.00001164936,0.001167555],"study_design_scores_gemma":[0.000002479763,0.00004066567,0.0002167503,0.000001103101,0.000003326099,0.00003958161,0.000003990589,0.0003982261,0.9990498,0.000007149783,0.0002337656,0.000003069156],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9794068,0.000833212,0.01809997,0.00009305276,0.00002079129,0.00005371263,0.0001724371,0.0002211248,0.001098847],"genre_scores_gemma":[0.9746724,0.0005801268,0.02098409,0.00006495875,0.00001241507,0.00007968695,0.0004424122,0.00008704362,0.003076809],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002086549,"threshold_uncertainty_score":0.01103485,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01105236901487816,"score_gpt":0.2578314400661127,"score_spread":0.2467790710512345,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}