{"id":"W4285891053","doi":"10.1016/j.xpro.2022.101554","title":"In situ detection of protein-protein interaction by proximity ligation assay in patient derived brain tumor stem cells","year":2022,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Jewish General Hospital; University of Ottawa","funders":"Canadian Institutes of Health Research; Cancer Research Society","keywords":"Proximity ligation assay; Stem cell; Ligation; In situ; Protocol (science); Computational biology; Chemistry; Biology; Cancer research; Cell biology; Molecular biology; Pathology; Medicine; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006910615,0.0001588677,0.0001771348,0.0001022613,0.00007109247,0.00001633884,0.0001463636,0.00008049606,0.00004148753],"category_scores_gemma":[0.00004385741,0.0001750794,0.00005918508,0.0002130969,0.00002942655,0.00002061492,0.000157897,0.0002701712,0.000003004284],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001428572,"about_ca_system_score_gemma":0.00009778684,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001153226,"about_ca_topic_score_gemma":0.0001815136,"domain_scores_codex":[0.9980741,0.0006473488,0.0004350013,0.0003783323,0.0002358833,0.0002292916],"domain_scores_gemma":[0.9993453,0.00001177812,0.0002987059,0.0002439963,0.00005740846,0.00004279991],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006483552,0.0002871334,0.00007248986,0.0001330651,0.000006850272,0.000005792383,0.0002497808,0.00005171432,0.9916735,0.00001074298,0.00005997998,0.006800598],"study_design_scores_gemma":[0.0009623491,0.001594893,0.00006070059,0.00008765073,0.000001293928,0.000004342069,0.0003232393,0.00009273527,0.9847205,0.000149355,0.01182219,0.0001807654],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9322073,0.0000187706,0.0004203235,0.00008505752,0.00003049075,0.06705539,0.00005266004,0.00001176191,0.0001182707],"genre_scores_gemma":[0.829922,2.474124e-7,0.0002308002,0.00007678333,0.00002265921,0.169599,0.0000350575,0.00001929612,0.00009422643],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1025436,"threshold_uncertainty_score":0.7139532,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01247772363953505,"score_gpt":0.2568475913801945,"score_spread":0.2443698677406594,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}