{"id":"W4286851645","doi":"10.3791/53153-v","title":"Imaging the Intracellular Trafficking of APP with Photoactivatable GFP","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"","keywords":"Endosome; Golgi apparatus; Green fluorescent protein; Amyloid precursor protein; Cell biology; Lysosome; Alpha secretase; Amyloid precursor protein secretase; Intracellular; LAMP1; Internalization; Senile plaques; Transport protein; Chemistry; Fluorescence microscope; Biology; Cell; Biochemistry; Alzheimer's disease; Fluorescence; Pathology; Medicine","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001728917,0.0002922422,0.0002131288,0.0002242684,0.0001989394,0.0003271966,0.0003887654,0.0006875896,0.001043597],"category_scores_gemma":[0.000104096,0.0002099443,0.0002661765,0.0002379268,0.0002214778,0.0003094618,0.0003365696,0.000962771,0.0004043157],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003671303,"about_ca_system_score_gemma":0.0002422153,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00108479,"about_ca_topic_score_gemma":0.0008721298,"domain_scores_codex":[0.999922,0.000006808091,0.00000318592,0.00002492829,0.00002053154,0.00002244016],"domain_scores_gemma":[0.9999397,0.00001409487,0.00001410167,0.000007172323,0.00001103685,0.00001394536],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002386745,0.00001184724,0.00004581442,0.00002138489,0.000001846017,0.00004951661,0.00001134186,0.00006925857,0.998561,0.000156545,0.00007616104,0.0009713373],"study_design_scores_gemma":[0.00001924892,0.0001543512,0.001892287,0.0000104113,0.00001177247,0.0004636265,0.00003434846,0.004808051,0.989277,0.0001502403,0.003168936,0.000009748245],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.9000874,0.001831104,0.08870149,0.0005970831,0.0001247242,0.0001440026,0.0006829245,0.0005361609,0.00729529],"genre_scores_gemma":[0.851614,0.003304978,0.1329313,0.0005470932,0.00005196104,0.000431101,0.0009100863,0.0001927351,0.01001674],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.00108479,"threshold_uncertainty_score":0.003491163,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01986018721030547,"score_gpt":0.3355578648770056,"score_spread":0.3156976776667001,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}