{"id":"W4286855377","doi":"10.3791/52687-v","title":"Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Neutrophil, Myeloperoxidase and Oxidative Mechanisms","field":"Immunology and Microbiology","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"","keywords":"Neutrophil extracellular traps; Immunofluorescence; Blot; Extracellular; Cell culture; A549 cell; DAPI; Biology; Staining; Chemistry; Cell biology; Molecular biology; In vitro; Immunology; Biochemistry; Inflammation; Gene; Antibody","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003672228,0.0007774475,0.0003667049,0.0003782353,0.0003167949,0.0003031696,0.0004474457,0.0004447938,0.004119874],"category_scores_gemma":[0.0002462504,0.0002338641,0.0002810495,0.0002299051,0.0001514238,0.000266784,0.0003778266,0.0007217055,0.002766755],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001605939,"about_ca_system_score_gemma":0.0003093088,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004647616,"about_ca_topic_score_gemma":0.001065428,"domain_scores_codex":[0.9996557,0.0000633707,0.00003808991,0.00006552913,0.0001325883,0.00004461375],"domain_scores_gemma":[0.999897,0.00001622582,0.00002160878,0.0000203122,0.00002530108,0.00001964392],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002119974,0.0000674375,0.0003069153,0.0002107238,0.00001264901,0.0001806275,0.00002824882,0.0003256479,0.9838638,0.0003640474,0.002232782,0.01219497],"study_design_scores_gemma":[0.0000942697,0.0007623881,0.005547126,0.0000843648,0.0000597976,0.002137617,0.00004909883,0.004022643,0.8939351,0.000563577,0.09269255,0.00005146406],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.4134881,0.02036759,0.5089669,0.001558322,0.00149061,0.006350411,0.02417092,0.003763088,0.01984399],"genre_scores_gemma":[0.4313839,0.01658571,0.4656338,0.0009164633,0.0004288288,0.00506768,0.04418193,0.0003424609,0.03545903],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004119874,"threshold_uncertainty_score":0.01378232,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05968336933729345,"score_gpt":0.390556859319232,"score_spread":0.3308734899819385,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}