{"id":"W4286856565","doi":"10.3791/55598-v","title":"Voltage-clamp Fluorometry in <em>Xenopus</em> Oocytes Using Fluorescent Unnatural Amino Acids","year":2017,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Electrochemical Analysis and Applications","field":"Chemistry","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"","keywords":"Xenopus; Fluorescence; Transfer RNA; Biophysics; Voltage clamp; Biochemistry; Chemistry; Amino acid; Intracellular; Biology; RNA; Membrane potential; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00104651,0.0007202739,0.0008958585,0.0005509009,0.000426691,0.0009582763,0.002667635,0.001013561,0.003001163],"category_scores_gemma":[0.0007441843,0.0005073739,0.0004806886,0.0005235271,0.001010006,0.001152546,0.0006425244,0.002117359,0.003121329],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001151734,"about_ca_system_score_gemma":0.0003177262,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001151236,"about_ca_topic_score_gemma":0.001428514,"domain_scores_codex":[0.9992114,0.0001018004,0.00009035769,0.0002929014,0.0002323623,0.00007107003],"domain_scores_gemma":[0.9996176,0.000113796,0.00007365881,0.00008691612,0.0000690665,0.00003889625],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002910888,0.00001568329,0.00006826084,0.00009452799,0.000007402366,0.00003410818,0.00002719054,0.00007527528,0.9948619,0.0009634428,0.0002769584,0.003546237],"study_design_scores_gemma":[0.00002554535,0.00005735383,0.001027484,0.00002934548,0.00002034999,0.0002465562,0.00002253883,0.003500063,0.9830824,0.0006306907,0.01133955,0.00001799595],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2164072,0.007945336,0.7454052,0.001436595,0.001065949,0.0005285171,0.004937603,0.006389644,0.01588405],"genre_scores_gemma":[0.4898057,0.01085968,0.4707139,0.00112413,0.0002695473,0.001625667,0.004051603,0.002789974,0.01875973],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003001163,"threshold_uncertainty_score":0.01003987,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02709970285862917,"score_gpt":0.37448014636632,"score_spread":0.3473804435076909,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}