{"id":"W4288695840","doi":"10.1101/2022.07.29.501992","title":"TraC of the conjugative plasmid pKM101 is secreted as a monomer in outer membrane vesicles and stimulates conjugation","year":2022,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Université de Montréal","keywords":"TRAC; Pilus; Complementation; Plasmid; Secretion; Cell biology; Cell; Bacterial conjugation; Escherichia coli; Chemistry; Type VI secretion system; Bacterial outer membrane; Cell membrane; Bacteria; Vesicle; Biology; Membrane; DNA; Biochemistry; Phenotype; Genetics; Gene; Virulence","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001544071,0.000293381,0.0002030197,0.000251546,0.0001642183,0.0005489115,0.0001752716,0.0003535783,0.001555227],"category_scores_gemma":[0.0002655977,0.0001869354,0.0002568367,0.0001954742,0.0002391919,0.0001805129,0.0003870493,0.000564907,0.0007430243],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003546848,"about_ca_system_score_gemma":0.0002119581,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004025773,"about_ca_topic_score_gemma":0.0004083962,"domain_scores_codex":[0.9998108,0.00003406593,0.00001259027,0.00002805446,0.00007163097,0.00004293477],"domain_scores_gemma":[0.9997995,0.00004942073,0.00003266789,0.00003227039,0.00001563773,0.00007045343],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001870737,0.00001198899,0.0001273829,0.000009398676,0.000001330387,0.00003998948,0.000008409425,0.0000164116,0.9993517,0.00006671995,0.0000200696,0.0003279345],"study_design_scores_gemma":[0.000005572869,0.00007260483,0.003598329,0.000004407732,0.000003973353,0.0002042087,0.00002492863,0.0006392592,0.9943563,0.00004082141,0.00104686,0.00000266683],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9962854,0.0003716282,0.001467718,0.0001006471,0.00002493579,0.00001244063,0.0001297668,0.00006002363,0.001547239],"genre_scores_gemma":[0.9928359,0.0001584296,0.001176633,0.00003851017,0.000009602903,0.00001574739,0.0005203045,0.00004191265,0.005202903],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001555227,"threshold_uncertainty_score":0.00520277,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008346892687515508,"score_gpt":0.2119799438644201,"score_spread":0.2036330511769046,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}