{"id":"W4289340044","doi":"10.1038/s42004-022-00711-6","title":"Microbial rhodoquinone biosynthesis proceeds via an atypical RquA-catalyzed amino transfer from S-adenosyl-L-methionine to ubiquinone","year":2022,"lang":"en","type":"article","venue":"Communications Chemistry","topic":"Metalloenzymes and iron-sulfur proteins","field":"Energy","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"Dalhousie University","funders":"Natural Sciences and Engineering Research Council of Canada; Killam Trusts; Dalhousie University; Nova Scotia Health Research Foundation; Howard Hughes Medical Institute","keywords":"Biosynthesis; Cofactor; Biochemistry; Enzyme; Chemistry; Methionine; Pyridoxal; Stereochemistry; Rhodospirillum rubrum; Methyltransferase; Mutant; Pyridoxal phosphate; Amino acid; DNA; Methylation","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow","insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.0004285487,0.0003826703,0.0004953692,0.00009511117,0.0009546575,0.00009095139,0.003103777,0.00021468,0.007362591],"category_scores_gemma":[0.0001194909,0.0004330439,0.0002208765,0.000667384,0.0002303203,0.0001799209,0.00125203,0.0008322943,0.0002004223],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002407897,"about_ca_system_score_gemma":0.0001218792,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001032173,"about_ca_topic_score_gemma":0.0002054952,"domain_scores_codex":[0.9974222,0.0003214106,0.0006946725,0.0006717903,0.0003974369,0.0004925341],"domain_scores_gemma":[0.9953508,0.0002134382,0.0001078637,0.003832723,0.0001317927,0.0003633249],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002142386,0.0008727681,0.0000382215,0.0000312429,0.0001104068,0.000003544796,0.0009176838,0.0001184118,0.9882891,0.0006935891,0.000668265,0.008042517],"study_design_scores_gemma":[0.000970167,0.00009153606,0.0008739446,0.0000338841,0.0001508466,0.00003972928,0.0007383066,0.0007514035,0.8395809,0.000412545,0.1556388,0.0007179196],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9861357,0.00104412,0.001808948,0.003768796,0.00009520155,0.0004982351,0.0002680495,0.0003206682,0.00606025],"genre_scores_gemma":[0.9807261,0.00009011888,0.0138045,0.0004592808,0.0002060919,0.0006082025,0.002052717,0.0001001357,0.00195284],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1549705,"threshold_uncertainty_score":0.9998121,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.029906814745837,"score_gpt":0.2579733403027183,"score_spread":0.2280665255568813,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}