{"id":"W4289524384","doi":"10.1111/pbi.13904","title":"<scp>CRISPR</scp>/Cas9‐mediated generation of non‐motile mutants to improve the harvesting efficiency of mass‐cultivated <i>Euglena gracilis</i>","year":2022,"lang":"en","type":"article","venue":"Plant Biotechnology Journal","topic":"Algal biology and biofuel production","field":"Energy","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"Innovation Cluster (Canada)","funders":"Japan Science and Technology Agency; Hanyang University","keywords":"Euglena gracilis; Biology; CRISPR; Mutant; Cas9; Genetics; Gene; Chloroplast","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003182027,0.0006338445,0.0005218814,0.0003509588,0.0003024501,0.0006973536,0.000791954,0.0007155795,0.003291939],"category_scores_gemma":[0.0002778033,0.000326194,0.0005119965,0.0003126462,0.0003325174,0.0002998029,0.0006672232,0.001401789,0.001679196],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005752833,"about_ca_system_score_gemma":0.0003503285,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0019222,"about_ca_topic_score_gemma":0.003270641,"domain_scores_codex":[0.9997044,0.00002666723,0.00004085894,0.00006429601,0.000112511,0.00005117397],"domain_scores_gemma":[0.9998273,0.00002643259,0.00005416483,0.00003089375,0.00002355651,0.00003763692],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004311345,0.00001807393,0.000104827,0.00008967441,0.000009870476,0.0001158817,0.00003021702,0.0001174951,0.9964429,0.0003654444,0.0004169297,0.002245581],"study_design_scores_gemma":[0.00003882452,0.0001071792,0.004053809,0.00002999394,0.00004380802,0.0004334232,0.00004723383,0.004599536,0.9649367,0.0001881483,0.02548181,0.00003946233],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8848009,0.002209813,0.07111014,0.0008809848,0.0005353387,0.0007934559,0.0155867,0.00683826,0.01724447],"genre_scores_gemma":[0.9274145,0.001008157,0.03462182,0.0005034492,0.00002261926,0.0004607265,0.01063885,0.001189024,0.02414079],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003291939,"threshold_uncertainty_score":0.01101261,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0156214736842781,"score_gpt":0.2176078489566624,"score_spread":0.2019863752723843,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}