{"id":"W4289946720","doi":"10.1021/acscatal.2c01956","title":"Active-Site Engineering Switches Carbohydrate Regiospecificity in a Fungal Copper Radical Oxidase","year":2022,"lang":"en","type":"article","venue":"ACS Catalysis","topic":"Photosynthetic Processes and Mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canada's Michael Smith Genome Sciences Centre; University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; Genome British Columbia; Genome Canada","keywords":"Copper; Chemistry; Active site; Glucose oxidase; Catalysis; Carbohydrate; Biochemistry; Combinatorial chemistry; Enzyme; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001770207,0.0001523209,0.0001831488,0.00009211448,0.00009689627,0.00001602118,0.000240307,0.00007090645,0.00003869446],"category_scores_gemma":[0.00006280556,0.0001618866,0.00009894049,0.0002969556,0.00002619976,0.000005616762,0.0002888148,0.0001838611,0.000004795615],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005686245,"about_ca_system_score_gemma":0.00006512536,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001012215,"about_ca_topic_score_gemma":0.00006184084,"domain_scores_codex":[0.9989455,0.00002771927,0.0001723456,0.0003952805,0.0001790342,0.0002800626],"domain_scores_gemma":[0.9994675,0.00001100255,0.00005640985,0.0003564128,0.00002291264,0.00008574213],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007893942,0.00009172952,0.0002835098,0.00001019575,0.00004941751,0.00003155276,0.0001311429,0.0006800586,0.9980864,0.00006804915,0.00008500744,0.0004039883],"study_design_scores_gemma":[0.0003409974,0.00008165563,0.0002989488,0.000003914561,0.00003360635,0.00002603642,0.0001508851,0.0003478427,0.9847825,0.00003177582,0.01368608,0.0002157528],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9988275,0.0002205515,0.000362417,0.0001787399,0.00008117285,0.0001071442,0.00005513638,0.00001752694,0.0001498835],"genre_scores_gemma":[0.9991132,0.00005216832,0.00007032044,0.0001648678,0.00006021816,0.0001140697,0.0002363821,0.00002444365,0.0001643283],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01360107,"threshold_uncertainty_score":0.6601546,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006442459501461597,"score_gpt":0.2012476445026804,"score_spread":0.1948051850012188,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}